Bovine mature adipocytes readily return to a proliferative state

S Wei; M.S Duarte; M Du; P.V.R Paulino; Z Jiang; E Albrecht; M Fernyhough-Culver; L Zan; G.J Hausman; M.V Dodson

doi:10.1016/j.tice.2012.08.001

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Bovine mature adipocytes readily return to a proliferative state

Journal article

Open access

Peer reviewed

Bovine mature adipocytes readily return to a proliferative state

S Wei, M.S Duarte, M Du, P.V.R Paulino, Z Jiang, E Albrecht, M Fernyhough-Culver, L Zan, G.J Hausman and M.V Dodson

Tissue & cell, Vol.44(6), pp.385-390

12/2012

DOI: https://doi.org/10.1016/j.tice.2012.08.001

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https://hdl.handle.net/2376/104890

PMID: 22943980

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Abstract

Mature adipocytes

Proliferation

DFAT cells

The dynamics of human and animal adipogenesis has been defined using several traditional cell systems including stromal vascular cells and adipocyte-related cell lines. But a relatively new cell system using progeny cells stemming from the dedifferentiation of purified cultures of mature adipocytes may be used for studying the development and biology of adipocytes. In this research, we show that isolated (and purified) mature adipocytes derived from Wagyu cattle dedifferentiate into progeny cells, and that these spindle-shaped, proliferative-competent daughter cells possess ability to proliferate. We outline the optimum cell culture system and offer precautionary thoughts for effective mature adipocyte culture. Collectively, this represents a novel cell model which may provide new insights into cell development, physiology and use as a model for animal production/composition, tissue engineering and disease treatment.

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Title: Bovine mature adipocytes readily return to a proliferative state
Creators: S Wei - College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China
M.S Duarte - Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA
M Du - Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA
P.V.R Paulino - Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil
Z Jiang - Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA
E Albrecht - Leibniz-Institute for Farm Animal Biology, Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany
M Fernyhough-Culver - The Hartz Mountain Corporation, Secaucus, NJ 07094, USA
L Zan - College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China
G.J Hausman - USDA-ARS, Richard B. Russell Agricultural Research Station, Athens, GA 30604, USA
M.V Dodson - Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA
Publication Details: Tissue & cell, Vol.44(6), pp.385-390
Academic Unit: Animal Sciences, Department of
Publisher: Elsevier Ltd
Identifiers: 99900546951501842
Language: English
Resource Type: Journal article