Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain

Jason R Gerstner; Quentin Z Bremer; William M Vander Heyden; Timothy M Lavaute; Jerry C Yin; Charles F Landry

doi:10.1371/journal.pone.0001631

Back

Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain

Journal article

Open access

Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain

Jason R Gerstner, Quentin Z Bremer, William M Vander Heyden, Timothy M Lavaute, Jerry C Yin and Charles F Landry

PloS one, Vol.3(2), pp.e1631-e1631

02/20/2008

DOI: https://doi.org/10.1371/journal.pone.0001631

Handle:

https://hdl.handle.net/2376/110532

PMCID: PMC2238817

PMID: 18286188

Files and links (1)

url

https://doi.org/10.1371/journal.pone.0001631View

Published (Version of record) Open

Abstract

Astrocytes - cytology

Neurons

Mice, Inbred C57BL

RNA, Messenger - analysis

Fatty Acid-Binding Protein 7

Circadian Rhythm

Hippocampus - cytology

Stem Cells - metabolism

Fatty Acid-Binding Proteins - genetics

Nerve Tissue Proteins - genetics

Hippocampus - metabolism

Animals

Gene Expression Regulation, Developmental

Mice

Astrocytes - metabolism

Brain fatty acid binding protein (Fabp7), which is important in early nervous system development, is expressed in astrocytes and neuronal cell precursors in mature brain. We report here that levels of Fabp7 mRNA in adult murine brain change over a 24 hour period. Unlike Fabp5, a fatty acid binding protein that is expressed widely in various cell types within brain, RNA analysis revealed that Fabp7 mRNA levels were elevated during the light period and lower during dark in brain regions involved in sleep and activity mechanisms. This pattern of Fabp7 mRNA expression was confirmed using in situ hybridization and found to occur throughout the entire brain. Changes in the intracellular distribution of Fabp7 mRNA were also evident over a 24 hour period. Diurnal changes in Fabp7, however, were not found in postnatal day 6 brain, when astrocytes are not yet mature. In contrast, granule cell precursors of the subgranular zone of adult hippocampus did undergo diurnal changes in Fabp7 expression. These changes paralleled oscillations in Fabp7 mRNA throughout the brain suggesting that cell-coordinated signals likely control brain-wide Fabp7 mRNA expression. Immunoblots revealed that Fabp7 protein levels also underwent diurnal changes in abundance, with peak levels occurring in the dark period. Of clock or clock-regulated genes, the synchronized, global cycling pattern of Fabp7 expression is unique and implicates glial cells in the response or modulation of activity and/or circadian rhythms.

Metrics

8 Record Views

See more details

Details

Title: Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain
Creators: Jason R Gerstner - Neuroscience Training Program, University of Wisconsin-Madison, Madison, Wisconsin, USA
Quentin Z Bremer
William M Vander Heyden
Timothy M Lavaute
Jerry C Yin
Charles F Landry
Publication Details: PloS one, Vol.3(2), pp.e1631-e1631
Academic Unit: Biomedical Sciences, Department of
Publisher: United States
Grant note: T32 GM007507 / NIGMS NIH HHS R01 DA019153 / NIDA NIH HHS 5 P50 CA084724-04 / NCI NIH HHS DA19153 / NIDA NIH HHS R01 MH067774 / NIMH NIH HHS P50 CA084724 / NCI NIH HHS
Identifiers: 99900547350601842
Language: English
Resource Type: Journal article