Journal article
Branched dimerization of Tat peptide improves permeability to HeLa and hippocampal neuronal cells
Chemical communications (Cambridge, England), Vol.51(25), pp.5463-5466
2015
Handle:
https://hdl.handle.net/2376/117654
PMID: 25733181
Abstract
A dimeric branched peptide TATp-D designed as an analogue of the HIV-Tat protein transduction domain (TATp), a prototypical cell penetrating peptide (CPP), demonstrates significantly enhanced cell uptake at 0.25 to 2.5 μM. Live cell confocal laser scanning microscopy revealed that multivalency dramatically improved the permeation potency of TATp-D to HeLa and primary hippocampal neuronal cells. The observed enhanced ability of TATp-D to translocate through the membrane is highlighted by a non-linear dependence on concentration, exhibiting the greatest uptake at sub-micromolar concentrations as compared to TATp. Multimerization via bis-Fmoc Lysine offered a synthetically straightforward method to investigate the effects of multivalent CPPs while offering orthogonal handles for cargo attachment, increasing the utility of CPPs at significantly lower concentrations.
Metrics
10 Record Views
Details
- Title
- Branched dimerization of Tat peptide improves permeability to HeLa and hippocampal neuronal cells
- Creators
- I. Abrrey Monreal - Department of Chemistry, Washington State University, Pullman, USAQian Liu - Paul G. Allen School for Global Animal Health, Washington State University, Pullman, USAKatherine Tyson - Department of Neuroscience, Washington State University, Pullman, USATyler Bland - Department of Neuroscience, Washington State University, Pullman, USADoralyn S Dalisay - Institute of Biological Chemistry, Washington State University, Pullman, USAErin V Adams - Department of Chemistry, Washington State University, Pullman, USAGary A Wayman - Department of Neuroscience, Washington State University, Pullman, USAHector C Aguilar - Paul G. Allen School for Global Animal Health, Washington State University, Pullman, USAJonel P Saludes - Department of Chemistry, Washington State University, Pullman, USA
- Publication Details
- Chemical communications (Cambridge, England), Vol.51(25), pp.5463-5466
- Academic Unit
- Paul G. Allen School for Global Animal Health; Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900548093801842
- Language
- English
- Resource Type
- Journal article