Journal article
Brassinosteroid Homeostasis in Arabidopsis Is Ensured by Feedback Expressions of Multiple Genes Involved in Its Metabolism
Plant physiology (Bethesda), Vol.138(2), pp.1117-1125
06/2005
Handle:
https://hdl.handle.net/2376/115939
PMCID: PMC1150425
PMID: 15908602
Abstract
Homeostasis of brassinosteroids (BRs) is essential for normal growth and development in higher plants. We examined responsiveness of 11 BR metabolic gene expressions to the decrease or increase of endogenous BR contents in Arabidopsis (
Arabidopsis thaliana
) to expand our knowledge of molecular mechanisms underlying BR homeostasis. Five BR-specific biosynthesis genes (
DET2
,
DWF4
,
CPD
,
BR6ox1
, and
ROT3
) and two sterol biosynthesis genes (
FK
and
DWF5
) were up-regulated in BR-depleted wild-type plants grown under brassinazole, a BR biosynthesis inhibitor. On the other hand, in BR-excessive wild-type plants that were fed with brassinolide, four BR-specific synthesis genes (
DWF4
,
CPD
,
BR6ox1
, and
ROT3
) and a sterol synthesis gene (
DWF7
) were down-regulated and a BR inactivation gene (
BAS1
) was up-regulated. However, their response to fluctuation of BR levels was highly reduced (
DWF4
) or nullified (the other eight genes) in a
bri1
mutant. Taken together, our results imply that BR homeostasis is maintained through feedback expressions of multiple genes, each of which is involved not only in BR-specific biosynthesis and inactivation, but also in sterol biosynthesis. Our results also indicate that their feedback expressions are under the control of a BRI1-mediated signaling pathway. Moreover, a weak response in the mutant suggests that
DWF4
alone is likely to be regulated in other way(s) in addition to BRI1 mediation.
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Details
- Title
- Brassinosteroid Homeostasis in Arabidopsis Is Ensured by Feedback Expressions of Multiple Genes Involved in Its Metabolism
- Creators
- Kiwamu Tanaka - Department of Agricultural Sciences and Natural Resources (K.T., S.O.), and Department of Biochemical Science and Technology (T.M.), Kagoshima University, Kagoshima 890–0065, Japan; Institute of Physical and Chemical Research, Wako, Saitama 351–0198, Japan (T.A., S.Y.); and Department of Food Science and Nutritional Health, Kyoto Prefectural University, Sakyo-ku, Kyoto 606–8522, Japan (Y.N.)Tadao Asami - Department of Agricultural Sciences and Natural Resources (K.T., S.O.), and Department of Biochemical Science and Technology (T.M.), Kagoshima University, Kagoshima 890–0065, Japan; Institute of Physical and Chemical Research, Wako, Saitama 351–0198, Japan (T.A., S.Y.); and Department of Food Science and Nutritional Health, Kyoto Prefectural University, Sakyo-ku, Kyoto 606–8522, Japan (Y.N.)Shigeo Yoshida - Department of Agricultural Sciences and Natural Resources (K.T., S.O.), and Department of Biochemical Science and Technology (T.M.), Kagoshima University, Kagoshima 890–0065, Japan; Institute of Physical and Chemical Research, Wako, Saitama 351–0198, Japan (T.A., S.Y.); and Department of Food Science and Nutritional Health, Kyoto Prefectural University, Sakyo-ku, Kyoto 606–8522, Japan (Y.N.)Yasushi Nakamura - Department of Agricultural Sciences and Natural Resources (K.T., S.O.), and Department of Biochemical Science and Technology (T.M.), Kagoshima University, Kagoshima 890–0065, Japan; Institute of Physical and Chemical Research, Wako, Saitama 351–0198, Japan (T.A., S.Y.); and Department of Food Science and Nutritional Health, Kyoto Prefectural University, Sakyo-ku, Kyoto 606–8522, Japan (Y.N.)Tomoaki Matsuo - Department of Agricultural Sciences and Natural Resources (K.T., S.O.), and Department of Biochemical Science and Technology (T.M.), Kagoshima University, Kagoshima 890–0065, Japan; Institute of Physical and Chemical Research, Wako, Saitama 351–0198, Japan (T.A., S.Y.); and Department of Food Science and Nutritional Health, Kyoto Prefectural University, Sakyo-ku, Kyoto 606–8522, Japan (Y.N.)Shigehisa Okamoto - Department of Agricultural Sciences and Natural Resources (K.T., S.O.), and Department of Biochemical Science and Technology (T.M.), Kagoshima University, Kagoshima 890–0065, Japan; Institute of Physical and Chemical Research, Wako, Saitama 351–0198, Japan (T.A., S.Y.); and Department of Food Science and Nutritional Health, Kyoto Prefectural University, Sakyo-ku, Kyoto 606–8522, Japan (Y.N.)
- Publication Details
- Plant physiology (Bethesda), Vol.138(2), pp.1117-1125
- Academic Unit
- Plant Pathology, Department of
- Publisher
- American Society of Plant Biologists
- Identifiers
- 99900548587601842
- Language
- English
- Resource Type
- Journal article