Journal article
Bromodeoxyuridine-Labeled Viral Particles as a Tool for Visualization of the Immediate-Early Events of Human Cytomegalovirus Infection
Journal of virology, Vol.78(14), pp.7818-7822
07/2004
Handle:
https://hdl.handle.net/2376/101753
PMCID: PMC434117
PMID: 15220456
Abstract
We describe here a simple method for labeling the genome of human cytomegalovirus, a large double-stranded DNA virus, with bromodeoxyuridine (BrdU). The labeled DNA was incorporated into viral particles, which were then collected in cell supernatant. To demonstrate the versatility and effectiveness of this method, labeled virions were used to study the immediate-early events of virus-host cell interaction via indirect immunofluorescence microscopy. It is our hope that this new methodology will prove useful in the study of binding, entry and viral genome deposition in diverse virus systems.
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Details
- Title
- Bromodeoxyuridine-Labeled Viral Particles as a Tool for Visualization of the Immediate-Early Events of Human Cytomegalovirus Infection
- Creators
- Kyle Rosenke - Department of Microbiology, Molecular Biology and Biochemistry and The Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3052Elizabeth A Fortunato - Department of Microbiology, Molecular Biology and Biochemistry and The Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3052
- Publication Details
- Journal of virology, Vol.78(14), pp.7818-7822
- Academic Unit
- UNKNOWN
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546572001842
- Language
- English
- Resource Type
- Journal article