Journal article
CAM Kinase IV Regulates Lineage Commitment and Survival of Erythroid Progenitors in a Non-Cell–Autonomous Manner
The Journal of cell biology, Vol.151(4), pp.811-824
11/13/2000
Handle:
https://hdl.handle.net/2376/108140
PMCID: PMC2169444
PMID: 11076966
Abstract
Developmental functions of calmodulin-dependent protein kinase IV (CaM KIV) have not been previously investigated. Here, we show that CaM KIV transcripts are widely distributed during embryogenesis and that strict regulation of CaM KIV activity is essential for normal primitive erythropoiesis. Xenopus embryos in which CaM KIV activity is either upregulated or inhibited show that hematopoietic precursors are properly specified, but few mature erythrocytes are generated. Distinct cellular defects underlie this loss of erythrocytes: inhibition of CaM KIV activity causes commitment of hematopoietic precursors to myeloid differentiation at the expense of erythroid differentiation, on the other hand, constitutive activation of CaM KIV induces erythroid precursors to undergo apoptotic cell death. These blood defects are observed even when CaM KIV activity is misregulated only in cells that do not contribute to the erythroid lineage. Thus, proper regulation of CaM KIV activity in nonhematopoietic tissues is essential for the generation of extrinsic signals that enable hematopoietic stem cell commitment to erythroid differentiation and that support the survival of erythroid precursors.
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Details
- Title
- CAM Kinase IV Regulates Lineage Commitment and Survival of Erythroid Progenitors in a Non-Cell–Autonomous Manner
- Creators
- Gary A Wayman - Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201-3098Melinda J Walters - Department of Cell and Developmental Biology, Oregon Health Sciences University, Portland, Oregon 97201-3098Kathryn Kolibaba - Department of Hematology, Oregon Health Sciences University, Portland, Oregon 97201-3098Thomas R Soderling - Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201-3098Jan L Christian - Department of Cell and Developmental Biology, Oregon Health Sciences University, Portland, Oregon 97201-3098
- Publication Details
- The Journal of cell biology, Vol.151(4), pp.811-824
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900547345601842
- Language
- English
- Resource Type
- Journal article