CD4 T Cell Antigens from Staphylococcus aureus Newman Strain Identified following Immunization with Heat-Killed Bacteria

Paulraj K Lawrence; Bachra Rokbi; Nadège Arnaud-Barbe; Eric L Sutten; Junzo Norimine; Kevin K Lahmers; Wendy C Brown

doi:10.1128/CVI.05642-11

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CD4 T Cell Antigens from Staphylococcus aureus Newman Strain Identified following Immunization with Heat-Killed Bacteria

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CD4 T Cell Antigens from Staphylococcus aureus Newman Strain Identified following Immunization with Heat-Killed Bacteria

Paulraj K Lawrence, Bachra Rokbi, Nadège Arnaud-Barbe, Eric L Sutten, Junzo Norimine, Kevin K Lahmers and Wendy C Brown

Clinical and vaccine immunology, Vol.19(4), pp.477-489

04/2012

DOI: https://doi.org/10.1128/CVI.05642-11

Handle:

https://hdl.handle.net/2376/112819

PMCID: PMC3318285

PMID: 22323557

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https://doi.org/10.1128/CVI.05642-11View

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Abstract

Vaccines

Staphylococcus aureus is a commensal bacterium associated with the skin and mucosal surfaces of humans and animals that can also cause chronic infection. The emergence of antibiotic-resistant strains such as methicillin-resistant S. aureus (MRSA) and strains causing chronic intramammary infections (IMI) in cows results in severe human and livestock infections. Conventional approaches to vaccine development have yielded only a few noneffective vaccines against MRSA or IMI strains, so there is a need for improved vaccine development. CD4 T lymphocytes are required for promoting gamma interferon (IFN-γ) mediated immunoglobulin isotype switching in B lymphocytes to produce high-affinity IgG antibodies and IFN-γ-mediated phagocyte activation for an effective resolution of bacterial infection. However, the lack of known CD4 T cell antigens from S. aureus has made it difficult to design effective vaccines. The goal of this study was to identify S. aureus proteins recognized by immune CD4 T cells. Using a reverse genetics approach, 43 antigens were selected from the S. aureus Newman strain. These included lipoproteins, proteases, transcription regulators, an alkaline shock protein, conserved-domain proteins, hemolysins, fibrinogen-binding protein, staphylokinase, exotoxin, enterotoxin, sortase, and protein A. Screening of expressed proteins for recall T cell responses in outbred, immune calves identified 13 proteins that share over 80% sequence identity among MRSA or IMI strains. These may be useful for inclusion in a broadly protective multiantigen vaccine against MRSA or IMI.

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Title: CD4 T Cell Antigens from Staphylococcus aureus Newman Strain Identified following Immunization with Heat-Killed Bacteria
Creators: Paulraj K Lawrence - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Bachra Rokbi - Sanofi Pasteur, Marcy l'Etoile, France
Nadège Arnaud-Barbe - Sanofi Pasteur, Marcy l'Etoile, France
Eric L Sutten - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Junzo Norimine - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Kevin K Lahmers - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Wendy C Brown - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Publication Details: Clinical and vaccine immunology, Vol.19(4), pp.477-489
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: American Society for Microbiology; 1752 N St., N.W., Washington, DC
Identifiers: 99900547556101842
Language: English
Resource Type: Journal article