Journal article
CD8 +/perforin +/WC1 − γδ T cells, not CD8 + αβ T cells, infiltrate vasculitis lesions of American bison ( Bison bison) with experimental sheep-associated malignant catarrhal fever
Veterinary immunology and immunopathology, Vol.136(3), pp.284-291
2010
Handle:
https://hdl.handle.net/2376/107501
Abstract
Sheep-associated malignant catarrhal fever (SA-MCF) caused by ovine herpesvirus-2 (OvHV-2), a γ-herpesvirus in the
Macavirus genus, is a fatal disease associated with lymphoproliferation, lymphocytic vasculitis, and mucosal ulceration in clinically susceptible species. SA-MCF is an important threat to American bison (
Bison bison) due to their high susceptibility to this disease. Currently, the pathogenesis of disease in SA-MCF is poorly understood, and the immunophenotype of lymphocytes that infiltrate the vascular lesions of bison and cattle with SA-MCF has been only partially defined. Previous single-color immunohistochemistry studies have demonstrated that CD8
+ cells and CD4
+ cells predominate within vascular infiltrates in cattle and bison. The CD8
+ cells detected in the vascular lesions of cattle and bison were assumed to be cytotoxic αβ T lymphocytes. However, polychromatic immunophenotyping analyses in this study showed that CD8
+/perforin
+ γδ T cells, CD4
+/perforin
− αβ T cells, and B cells infiltrate vascular lesions in the urinary bladder, kidney, and liver of six bison with experimentally-induced SA-MCF. CD8
+ αβ T cells and WC1
+ γδ T cell cells were only infrequently and inconsistently identified. This study confirmed our hypothesis that the predominant CD8
+ lymphocytes infiltrating the vascular lesions of bison with SA-MCF are cytotoxic lymphocytes of the innate immune system, not CD8
+ αβ T cells. Results of the present study support the previous suggestions that MCF is fundamentally a disease of immune dysregulation.
Metrics
20 Record Views
Details
- Title
- CD8 +/perforin +/WC1 − γδ T cells, not CD8 + αβ T cells, infiltrate vasculitis lesions of American bison ( Bison bison) with experimental sheep-associated malignant catarrhal fever
- Creators
- Danielle D Nelson - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United StatesWilliam C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United StatesWendy C Brown - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United StatesHong Li - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United StatesDonal O’Toole - University of Wyoming, Laramie, WY, United StatesJ. Lindsay Oaks - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United States
- Publication Details
- Veterinary immunology and immunopathology, Vol.136(3), pp.284-291
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546702501842
- Language
- English
- Resource Type
- Journal article