Journal article
CONTROL OF FIBRONECTIN SYNTHESIS BY RAT GRANULOSA CELLS IN CULTURE
Endocrinology (Philadelphia), Vol.115(5), pp.2029-2031
11/1984
Handle:
https://hdl.handle.net/2376/113464
PMID: 6489268
Abstract
The secreted and cellular [35S]methionine-radiolabeled proteins of cultured rat granulosa cells were separated by electrophoresis on sodium dodecylsulfate (SDS) polyacrylamide gradient slab gels. From 24 to 72 h of culture FSH increased the intensity of labeling of most of the secreted proteins. A 220,000-dalton protein, however, increased in intensity only in control cultures and became the major secreted protein after 72 h, comprising 20% of the total radiolabeled proteins. This protein was identified as fibronectin by immunoprecipitation. There was no increase in the secreted or cellular fibronectin in FSH- or testosterone- and insulin-treated cultures. These studies indicate that a component of extracellular matrix is a major secretory product of unstimulated immature granulosa cells. As hormones induce the differentiated functions of granulosa cells in culture, the secretion of fibronectin is inhibited.
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Details
- Title
- CONTROL OF FIBRONECTIN SYNTHESIS BY RAT GRANULOSA CELLS IN CULTURE
- Creators
- MICHAEL K SKINNERJENNIFER H DORRINGTON
- Publication Details
- Endocrinology (Philadelphia), Vol.115(5), pp.2029-2031
- Academic Unit
- Biological Sciences, School of
- Identifiers
- 99900548221701842
- Language
- English
- Resource Type
- Journal article