CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins

Chungwon Chung; Robert H Mealey; Travis C McGuire

doi:10.1016/j.virol.2004.06.035

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CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins

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CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins

Chungwon Chung, Robert H Mealey and Travis C McGuire

Virology (New York, N.Y.), Vol.327(1), pp.144-154

09/15/2004

DOI: https://doi.org/10.1016/j.virol.2004.06.035

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https://hdl.handle.net/2376/108539

PMCID: PMC3342308

PMID: 15327905

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https://doi.org/10.1016/j.virol.2004.06.035View

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Abstract

T-Lymphocytes, Cytotoxic - immunology

Amino Acid Sequence

Carrier State - immunology

Epitope Mapping

Gene Products, gag - immunology

Molecular Sequence Data

Epitopes, T-Lymphocyte - genetics

Histocompatibility Antigens Class I - genetics

Infectious Anemia Virus, Equine - immunology

Infectious Anemia Virus, Equine - genetics

Gene Products, gag - chemistry

Infectious Anemia Virus, Equine - pathogenicity

Carrier State - virology

Animals

Infectious Anemia Virus, Equine - classification

Viral Matrix Proteins - chemistry

Alleles

Capsid Proteins - chemistry

Capsid Proteins - immunology

Horses

Equine Infectious Anemia - immunology

Viral Matrix Proteins - immunology

Cytotoxic T lymphocytes (CTL) are important for controlling equine infectious anemia virus (EIAV). Because Gag matrix (MA) and capsid (CA) are the most frequently recognized proteins, the hypothesis that CTL from EIAV-infected horses with diverse MHC class I alleles recognize epitope clusters (EC) in these proteins was tested. Four EC were identified by CTL from 15 horses and 8 of these horses had diverse MHC class I alleles. Two of the eight had CTL to EC1, six to EC2, five to EC3, and four to EC4. Because EC2-4 were recognized by CTL from >50% of horses with diverse alleles, the hypothesis was accepted. EC1 and EC3 were the most conserved EC and these more conserved broadly recognized EC may be most useful for CTL induction, helping overcome MHC class I polymorphism and antigenic variation.

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Title: CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins
Creators: Chungwon Chung - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99165-7040, USA
Robert H Mealey
Travis C McGuire
Publication Details: Virology (New York, N.Y.), Vol.327(1), pp.144-154
Academic Unit: Veterinary Clinical Sciences, Department of
Publisher: United States
Grant note: AI01575 / NIAID NIH HHS AI47660 / NIAID NIH HHS K08 AI001575 / NIAID NIH HHS AI24291 / NIAID NIH HHS
Identifiers: 99900547395101842
Language: English
Resource Type: Journal article