Journal article
Cadmium‐induced cell death in BY‐2 cell culture starts with vacuolization of cytoplasm and terminates with necrosis
Physiologia plantarum, Vol.151(4), pp.423-433
08/2014
Handle:
https://hdl.handle.net/2376/112091
PMID: 24359567
Abstract
Cadmium is a potent inducer of programmed cell death (PCD) in plants but the morphological changes in cells exposed to cadmium are poorly characterized. Using light and transmission electron microscopy (TEM) we have investigated the changes in ultrastructure of tobacco BY‐2 cells treated with 50 µM CdSO4. The cadmium‐induced alterations in cell morphology occurred gradually over a period of 3–4 days and the first stages of the response resembled vacuolar type of cell death. The initial formation of numerous small cytoplasmic vacuoles and dilation of endoplasmic reticulum was followed first by fusion of smaller vacuoles with each other and with big vacuoles, and then by the appearance of autophagic vacuoles containing autophagic bodies. The final stages of cell death were accompanied by necrotic features including loss of plasmalemma integrity, shrinkage of the protoplast and unprocessed cellular components. In addition, we observed a gradual degradation of nuclear material. Our results demonstrate that cadmium‐induced plant cell death is a slow process featuring elements of vacuolar cell death and terminating with necrosis.
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Details
- Title
- Cadmium‐induced cell death in BY‐2 cell culture starts with vacuolization of cytoplasm and terminates with necrosis
- Creators
- Jaromir Kutik - Charles University in PragueAndrea Kuthanova - Charles University in PragueAndrei Smertenko - Queen's University BelfastLukas Fischer - Charles University in PragueZdenek Opatrny - Charles University in Prague
- Publication Details
- Physiologia plantarum, Vol.151(4), pp.423-433
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- Number of pages
- 11
- Grant note
- Charles University in Prague (SVV 265203/2012) Ministry of Education, Youth and Sports of the Czech Republic (MSM0021620858; LC06034)
- Identifiers
- 99900547768101842
- Language
- English
- Resource Type
- Journal article