Journal article
Calcitonin Gene-Related Peptide Partially Reverses Decreased Production of Chemokines KC and MIP-2 Following Murine Sepsis
Inflammation, Vol.26(4), pp.167-174
08/2002
Handle:
https://hdl.handle.net/2376/116612
PMID: 12184630
Abstract
The secretion of calcitonin gene-related peptide (CGRP) and the chemokines KC and MIP-2 are increased in the animal models of endotoxemic and septic shock. We tested whether CGRP could modulate KC and MIP-2 secretion from different sources of macrophages after murine sepsis induced by cecal ligation and puncture (CLP). Macrophages were obtained from the peritoneal exudate and lung of female BALB/c mice 16 h after CLP and plated in culture with CGRP and/or LPS for 12 h. The results showed that peritoneal macrophage production of the chemokines (KC, MIP-2) and cytokines (TNF-α, IL-6) was markedly decreased in CLP mice. Alveolar macrophages did not display decreased cytokine/chemokines production after CLP. CGRP (0.1 nM–10 nM) partially reversed this decreased production of LPS-induced KC and MIP-2 from peritoneal macrophages. These results suggest that CGRP might be intimately involved in recruitment of neutrophils by promoting local production of the chemokines KC and MIP-2 in murine sepsis.
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Details
- Title
- Calcitonin Gene-Related Peptide Partially Reverses Decreased Production of Chemokines KC and MIP-2 Following Murine Sepsis
- Creators
- Xian Wang - Department of Physiology Peking University, Health Science Center Beijing 100083 P. R. ChinaSamuel Ebong - Department of Pathology University of Michigan Medical School Ann Arbor MI 48109Douglas Call - Department of Pathology University of Michigan Medical School Ann Arbor MI 48109David Newcomb - Department of Pathology University of Michigan Medical School Ann Arbor MI 48109Gerald Bolgos - Department of Pathology University of Michigan Medical School Ann Arbor MI 48109Daniel Remick - Department of Pathology University of Michigan Medical School Ann Arbor MI 48109
- Publication Details
- Inflammation, Vol.26(4), pp.167-174
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- Kluwer Academic Publishers-Plenum Publishers; New York
- Identifiers
- 99900547900801842
- Language
- English
- Resource Type
- Journal article