Journal article
Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors
Development (Cambridge), Vol.129(6), pp.1455-1466
03/2002
Handle:
https://hdl.handle.net/2376/113723
PMID: 11880354
Abstract
In the current study, we show that bone morphogenetic proteins (BMPs) play a role in hematopoiesis that is independent of their function in specifying ventral mesodermal fate. When BMP activity is upregulated or inhibited in Xenopus embryos hematopoietic precursors are specified properly but few mature erythrocytes are generated. Distinct cellular defects underlie this loss of erythrocytes: inhibition of BMP activity induces erythroid precursors to undergo apoptotic cell death, whereas constitutive activation of BMPs causes an increase in commitment of hematopoietic progenitors to myeloid differentiation and a concomitant decrease in erythrocytes that is not due to enhanced apoptosis. These blood defects are observed even when BMP activity is misregulated solely in non-hematopoietic (ectodermal) cells, demonstrating that BMPs generate extrinsic signals that regulate hematopoiesis independent of mesodermal patterning. Further analysis revealed that endogenous calmodulin-dependent protein kinase IV (CaM KIV) is required to negatively modulate hematopoietic functions of BMPs downstream of receptor activation. Our data are consistent with a model in which CaM KIV inhibits BMP signals by activating a substrate, possibly cAMP-response element-binding protein (CREB), that recruits limiting amounts of CREB binding protein (CBP) away from transcriptional complexes functioning downstream of BMPs.
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Details
- Title
- Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors
- Creators
- Melinda J Walters - Department of Cell and Developmental Biology, Oregon Health Sciences University, School of Medicine, 3181 SW Sam Jackson Park Road, Portland, OR 97201-3098, USAGary A WaymanJohn C NotisRichard H GoodmanThomas R SoderlingJan L Christian
- Publication Details
- Development (Cambridge), Vol.129(6), pp.1455-1466
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- England
- Grant note
- DK45423 / NIDDK NIH HHS HD01167 / NICHD NIH HHS DK44239 / NIDDK NIH HHS
- Identifiers
- 99900547537001842
- Language
- English
- Resource Type
- Journal article