Campylobacter jejuni Strains Compete for Colonization in Broiler Chicks

Michael E Konkel; Jeffrey E Christensen; A. Singh Dhillon; Alison B Lane; Rebekah Hare-Sanford; Dennis M Schaberg; Charles L Larson

doi:10.1128/AEM.02193-06

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Campylobacter jejuni Strains Compete for Colonization in Broiler Chicks

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Campylobacter jejuni Strains Compete for Colonization in Broiler Chicks

Michael E Konkel, Jeffrey E Christensen, A. Singh Dhillon, Alison B Lane, Rebekah Hare-Sanford, Dennis M Schaberg and Charles L Larson

Applied and environmental microbiology, Vol.73(7), pp.2297-2305

04/2007

DOI: https://doi.org/10.1128/AEM.02193-06

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https://hdl.handle.net/2376/116493

PMCID: PMC1855682

PMID: 17293510

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Abstract

Microbial Ecology

Campylobacter jejuni isolates possess multiple adhesive proteins termed adhesins, which promote the organism's attachment to epithelial cells. Based on the proposal that one or more adhesins are shared among C. jejuni isolates, we hypothesized that C. jejuni strains would compete for intestinal and cecal colonization in broiler chicks. To test this hypothesis, we selected two C. jejuni strains with unique SmaI pulsed-field gel electrophoresis macrorestriction profiles and generated one nalidixic acid-resistant strain (the F38011 Nal r strain) and one streptomycin-resistant strain (the 02-833L Str r strain). In vitro binding assays revealed that the C. jejuni F38011 Nal r and 02-833L Str r strains adhered to LMH chicken hepatocellular carcinoma epithelial cells and that neither strain influenced the binding potential of the other strain at low inoculation doses. However, an increase in the dose of the C. jejuni 02-833L Str r strain relative to that of the C. jejuni F38011 Nal r strain competitively inhibited the binding of the C. jejuni F38011 Nal r strain to LMH cells in a dose-dependent fashion. Similarly, the C. jejuni 02-833L Str r strain was found to significantly reduce the efficiency of intestinal and cecal colonization by the C. jejuni F38011 Nal r strain in broiler chickens. Based on the number of bacteria recovered from the ceca, the maximum number of bacteria that can colonize the digestive tracts of chickens may be limited by host constraints. Collectively, these data support the hypothesis that C. jejuni strains compete for colonization in chicks and suggest that it may be possible to design novel intervention strategies for reducing the level at which C. jejuni colonizes the cecum.

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Title: Campylobacter jejuni Strains Compete for Colonization in Broiler Chicks
Creators: Michael E Konkel - School of Molecular Biosciences, Washington State University, Pullman, Washington
Jeffrey E Christensen - School of Molecular Biosciences, Washington State University, Pullman, Washington
A. Singh Dhillon - School of Molecular Biosciences, Washington State University, Pullman, Washington
Alison B Lane - School of Molecular Biosciences, Washington State University, Pullman, Washington
Rebekah Hare-Sanford - School of Molecular Biosciences, Washington State University, Pullman, Washington
Dennis M Schaberg - School of Molecular Biosciences, Washington State University, Pullman, Washington
Charles L Larson - School of Molecular Biosciences, Washington State University, Pullman, Washington
Publication Details: Applied and environmental microbiology, Vol.73(7), pp.2297-2305
Academic Unit: Molecular Biosciences, School of; Washington Animal Disease Diagnostic Laboratory
Publisher: American Society for Microbiology
Identifiers: 99900548306801842
Language: English
Resource Type: Journal article