Campylobacter jejuni survival within human epithelial cells is enhanced by the secreted protein CiaI

Daelynn R Buelow; Jeffrey E Christensen; Jason M Neal-McKinney; Michael E Konkel

doi:10.1111/j.1365-2958.2011.07645.x

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Campylobacter jejuni survival within human epithelial cells is enhanced by the secreted protein CiaI

Journal article

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Campylobacter jejuni survival within human epithelial cells is enhanced by the secreted protein CiaI

Daelynn R Buelow, Jeffrey E Christensen, Jason M Neal-McKinney and Michael E Konkel

Molecular microbiology, Vol.80(5), pp.1296-1312

06/2011

DOI: https://doi.org/10.1111/j.1365-2958.2011.07645.x

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https://hdl.handle.net/2376/109798

PMCID: PMC3103613

PMID: 21435039

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https://doi.org/10.1111/j.1365-2958.2011.07645.xView

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Abstract

Campylobacter jejuni - growth & development

Epithelial Cells - microbiology

Humans

Bacterial Proteins - genetics

Campylobacter jejuni - metabolism

Bacterial Proteins - metabolism

Microbial Viability

Campylobacter Infections - microbiology

Gene Expression Regulation, Bacterial

Campylobacter jejuni - genetics

Protein Transport

Although it is known that Campylobacter jejuni invade the cells that line the human intestinal tract, the bacterial proteins that enable this pathogen to survive within Campylobacter-containing vacuoles (CCV) have not been identified. Here, we describe the identification and characterization of a protein that we termed CiaI for Campylobacter invasion antigen involved in intracellular survival. We show that CiaI harbours an amino-terminal type III secretion sequence and is secreted from C. jejuni through the flagellar type III secretion system. In addition, the ciaI mutant was impaired in intracellular survival when compared with a wild-type strain, as judged by the gentamicin-protection assay. Fluorescence microscopy examination of epithelial cells infected with the C. jejuni ciaI mutant revealed that the CCV were more frequently co-localized with Cathepsin D (a lysosomal marker) than the CCV in cells infected with a C. jejuni wild-type strain. Ectopic expression of CiaI-GFP in epithelial cells yielded a punctate phenotype not observed with the other C. jejuni genes, and this phenotype was abolished by mutation of a dileucine motif located in the carboxy-terminus of the protein. Based on the data, we conclude that CiaI contributes to the ability of C. jejuni to survive within epithelial cells.

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Title: Campylobacter jejuni survival within human epithelial cells is enhanced by the secreted protein CiaI
Creators: Daelynn R Buelow - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7520, USA
Jeffrey E Christensen
Jason M Neal-McKinney
Michael E Konkel
Publication Details: Molecular microbiology, Vol.80(5), pp.1296-1312
Academic Unit: Molecular Biosciences, School of
Publisher: England
Grant note: N01-AL-30055 / PHS HHS T32 GM083864-03 / NIGMS NIH HHS N01 AI030055 / NIAID NIH HHS T32 GM083864 / NIGMS NIH HHS
Identifiers: 99900547222901842
Language: English
Resource Type: Journal article