Journal article
Canine ABCB4: Tissue expression and cDNA structure
Research in veterinary science, Vol.89(1), pp.65-71
2010
Handle:
https://hdl.handle.net/2376/105618
PMID: 20153493
Abstract
The ABCB gene subfamily of ABC (ATP-binding cassette) transporters is responsible for transporting a wide spectrum of molecules including peptides, iron, bile salts, drugs, and phospholipids. In humans, ABCB4 appears to be exclusively expressed on the apical membrane of hepatocytes where it translocates phosphatidylcholine from the inner to the outer leaflet of the canalicular membrane. Functional alterations in the ABCB4 transporter are associated with a number of cholestatic syndromes in humans. Because of its role in biliary lipid homeostasis in humans, investigation of the
ABCB4 gene in dogs is warranted. Thus, the full cDNA sequence of canine
ABCB4 was elucidated and its mRNA and protein expression levels in tissues were determined. Canine
ABCB4 consists of 3804 nucleotides spanning 26 exons and is 89% identical to human ABCB4. Expression of ABCB4 in canine liver supports a potential role for the protein in normal biliary function similar to that in humans. The function of ABCB4 expressed in brain tissue has yet to be determined.
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Details
- Title
- Canine ABCB4: Tissue expression and cDNA structure
- Creators
- Erick S SpencerJonathan MinchKevin K LahmersGary J HaldorsonKatrina L Mealey
- Publication Details
- Research in veterinary science, Vol.89(1), pp.65-71
- Academic Unit
- Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier India Pvt Ltd
- Identifiers
- 99900546563001842
- Language
- English
- Resource Type
- Journal article