Carriage of stx2a differentiates clinical and bovine-biased strains of Escherichia coli O157

Smriti Shringi; Carrie Schmidt; Kaya Katherine; Kelly A Brayton; Dale D Hancock; Thomas E Besser

doi:10.1371/journal.pone.0051572

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Carriage of stx2a differentiates clinical and bovine-biased strains of Escherichia coli O157

Journal article

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Carriage of stx2a differentiates clinical and bovine-biased strains of Escherichia coli O157

Smriti Shringi, Carrie Schmidt, Kaya Katherine, Kelly A Brayton, Dale D Hancock and Thomas E Besser

PloS one, Vol.7(12), pp.e51572-e51572

2012

DOI: https://doi.org/10.1371/journal.pone.0051572

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https://hdl.handle.net/2376/109580

PMCID: PMC3519850

PMID: 23240045

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Abstract

Escherichia coli O157 - genetics

Humans

Shiga Toxin 2 - metabolism

Shiga Toxin 2 - genetics

Escherichia coli Infections - genetics

Escherichia coli O157 - pathogenicity

Shiga Toxin - genetics

Animals

Shiga Toxin - metabolism

Virulence - genetics

Cattle

Bacteriophages - metabolism

Bacteriophages - genetics

Shiga toxin (Stx) are cardinal virulence factors of enterohemorrhagic E. coli O157:H7 (EHEC O157). The gene content and genomic insertion sites of Stx-associated bacteriophages differentiate clinical genotypes of EHEC O157 (CG, typical of clinical isolates) from bovine-biased genotypes (BBG, rarely identified among clinical isolates). This project was designed to identify bacteriophage-mediated differences that may affect the virulence of CG and BBG. Stx-associated bacteriophage differences were identified by whole genome optical scans and characterized among >400 EHEC O157 clinical and cattle isolates by PCR. Optical restriction maps of BBG strains consistently differed from those of CG strains only in the chromosomal insertion sites of Stx2-associated bacteriophages. Multiplex PCRs (stx1, stx2a, and stx2c as well as Stx-associated bacteriophage-chromosomal insertion site junctions) revealed four CG and three BBG that accounted for >90% of isolates. All BBG contained stx2c and Stx2c-associated bacteriophage-sbcB junctions. All CG contained stx2a and Stx2a-associated bacteriophage junctions in wrbA or argW. Presence or absence of stx2a (or another product encoded by the Stx2a-associated bacteriophage) is a parsimonious explanation for differential virulence of BBG and CG, as reflected in the distributions of these genotypes in humans and in the cattle reservoir.

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Title: Carriage of stx2a differentiates clinical and bovine-biased strains of Escherichia coli O157
Creators: Smriti Shringi - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America
Carrie Schmidt
Kaya Katherine
Kelly A Brayton
Dale D Hancock
Thomas E Besser
Publication Details: PloS one, Vol.7(12), pp.e51572-e51572
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: T32 AI007025 / NIAID NIH HHS N01AI30055 / NIAID NIH HHS
Identifiers: 99900547027301842
Language: English
Resource Type: Journal article