Catalase activity is necessary for heat-shock recovery in Aspergillus nidulans germlings

Maria Antônia Noventa-Jordão; Ricardo M Couto; Maria Helena S Goldman; Jesus Aguirre; Suresh Iyer; Allan Caplan; Hector F Terenzi; Gustavo H Goldman

doi:10.1099/00221287-145-11-3229

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Catalase activity is necessary for heat-shock recovery in Aspergillus nidulans germlings

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Catalase activity is necessary for heat-shock recovery in Aspergillus nidulans germlings

Maria Antônia Noventa-Jordão, Ricardo M Couto, Maria Helena S Goldman, Jesus Aguirre, Suresh Iyer, Allan Caplan, Hector F Terenzi and Gustavo H Goldman

Microbiology (Society for General Microbiology), Vol.145 ( Pt 11)(11), pp.3229-3234

11/1999

DOI: https://doi.org/10.1099/00221287-145-11-3229

Handle:

https://hdl.handle.net/2376/115913

PMID: 10589732

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Abstract

RNA, Fungal - analysis

Aspergillus nidulans - physiology

Catalase - genetics

Aspergillus nidulans - drug effects

Heat-Shock Proteins - metabolism

Hydrogen Peroxide - pharmacology

RNA, Messenger - analysis

Chromatography, Gas

Fungal Proteins - genetics

Catalase - metabolism

Heat-Shock Proteins - genetics

Blotting, Northern

Trehalose - metabolism

Time Factors

Gene Deletion

Mannitol - metabolism

Aspergillus nidulans - enzymology

Fungal Proteins - metabolism

To understand the molecular mechanisms induced by stress that contribute to the development of tolerance in eukaryotic cells, the filamentous fungus Aspergillus nidulans has been chosen as a model system. Here, the response of A. nidulans germlings to heat shock is reported. The heat treatment dramatically increased the concentration of trehalose and induced the accumulation of mannitol and mRNA from the catalase gene catA. Both mannitol and catalase function to protect cells from different reactive oxygen species. Treatment with hydrogen peroxide increased A. nidulans germling viability after heat shock whilst mutants deficient in catalase were more sensitive to a 50 degrees C heat exposure. It is concluded that the defence against the lethal effects of heat exposure can be correlated with the activity of the defence system against oxidative stress.

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Title: Catalase activity is necessary for heat-shock recovery in Aspergillus nidulans germlings
Creators: Maria Antônia Noventa-Jordão - Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo and Universidade de Franca, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil1
Ricardo M Couto - Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo and Universidade de Franca, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil1
Maria Helena S Goldman - Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil2
Jesus Aguirre - Instituto de Fisiologia Celular-UNAM, Mexico City, Mexico3
Suresh Iyer - Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID, USA4
Allan Caplan - Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID, USA4
Hector F Terenzi - Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil2
Gustavo H Goldman - Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo and Universidade de Franca, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil1
Publication Details: Microbiology (Society for General Microbiology), Vol.145 ( Pt 11)(11), pp.3229-3234
Academic Unit: UNKNOWN
Publisher: England
Identifiers: 99900548323701842
Language: English
Resource Type: Journal article