Journal article
Catalytic Mechanism of 5-Chlorohydroxyhydroquinone Dehydrochlorinase from the YCII Superfamily of Largely Unknown Function
The Journal of biological chemistry, Vol.288(40), pp.28447-28456
10/04/2013
Handle:
https://hdl.handle.net/2376/117030
PMCID: PMC3789946
PMID: 23955343
Abstract
Background:
TftG is a YCII superfamily dehydrochlorinase that catalyzes conversion of 5-chlorohydroxyhydroquinone to hydroxybenzoquinone.
Results:
The TftG crystal structure in complex with product analog 2,5-dihydroxybenzoquinone illustrated the catalytic residues and mechanism.
Conclusion:
A His-Asp dyad and other conserved signature residues are implicated for catalysis and substrate binding.
Significance:
This is the first elucidation of a YCII superfamily protein mechanism, which helps explain their obscure nature.
TftG, 5-chloro-2-hydroxyhydroquinone (5-CHQ) dehydrochlorinase, is involved in the biodegradation of 2,4,5-trichlorophenoxyacetate by
Burkholderia phenoliruptrix
AC1100. It belongs to the YCII superfamily, a group of proteins with largely unknown function. In this work, we utilized structural and functional studies, including the apo-form and 2,5-dihydroxybenzoquinone binary complex crystal structures, computational analysis, and site-directed mutagenesis, to determine the dehydrochlorination mechanism. The His-Asp dyad, which initiates catalysis, is strongly conserved in YCII-like proteins. In addition, other catalytically important residues such as Pro-76, which orients the His-Asp catalytic dyad; Arg-17 and Ser-56, which form an oxyanion hole; and Asp-9, which stabilizes the oxyanion hole, are among the most highly conserved residues across the YCII superfamily members. The comprehensive characterization of TftG helps not only for identifying effective mechanisms for chloroaromatic dechlorination but also for understanding the functions of YCII superfamily members, which we propose to be lyases.
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Details
- Title
- Catalytic Mechanism of 5-Chlorohydroxyhydroquinone Dehydrochlorinase from the YCII Superfamily of Largely Unknown Function
- Creators
- Robert P Hayes - From theKevin M Lewis - From theLuying Xun - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164ChulHee Kang - From the
- Publication Details
- The Journal of biological chemistry, Vol.288(40), pp.28447-28456
- Academic Unit
- Chemistry, Department of; Molecular Biosciences, School of
- Publisher
- American Society for Biochemistry and Molecular Biology; 9650 Rockville Pike, Bethesda, MD 20814, U.S.A
- Identifiers
- 99900547722301842
- Language
- English
- Resource Type
- Journal article