Journal article
Cationic isotachophoresis separation of the biomarker cardiac troponin I from a high‐abundance contaminant, serum albumin
Electrophoresis, Vol.35(14), pp.2029-2038
07/2014
Handle:
https://hdl.handle.net/2376/104746
PMCID: PMC4230826
PMID: 24723384
Abstract
Cationic ITP was used to separate and concentrate fluorescently tagged cardiac troponin I (cTnI) from two proteins with similar isoelectric properties in a PMMA straight‐channel microfluidic chip. In an initial set of experiments, cTnI was effectively separated from R‐Phycoerythrin using cationic ITP in a pH 8 buffer system. Then, a second set of experiments was conducted in which cTnI was separated from a serum contaminant, albumin. Each experiment took ∼10 min or less at low electric field strengths (34 V/cm) and demonstrated that cationic ITP could be used as an on‐chip removal technique to isolate cTnI from albumin. In addition to the experimental work, a 1D numerical simulation of our cationic ITP experiments has been included to qualitatively validate experimental observations.
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Details
- Title
- Cationic isotachophoresis separation of the biomarker cardiac troponin I from a high‐abundance contaminant, serum albumin
- Creators
- Thomas Jacroux - Washington State UniversityDanny Bottenus - Washington State UniversityBennett Rieck - Washington State UniversityCornelius F Ivory - Washington State UniversityWen‐ji Dong - Pharmacology and Physiology, Washington State University
- Publication Details
- Electrophoresis, Vol.35(14), pp.2029-2038
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Number of pages
- 10
- Grant note
- Washington State University National Institutes of Health Protein Biotechnology Training Program Award (T32GM008336) Life Science Discovery Fund National Institutes of Health Grants (HL80186; HL80186‐5S1)
- Identifiers
- 99900546635801842
- Language
- English
- Resource Type
- Journal article