Journal article
CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin
Biosensors & bioelectronics, Vol.26(3), pp.1109-1113
2010
Handle:
https://hdl.handle.net/2376/110507
PMID: 20850960
Abstract
A CdSe/ZnS quantum dot (QD) based electrochemical immunoassay of phosphorylated bovine serum albumin (BSA-OP) as a protein biomarker is presented. The QDs were used as labels for amplifying electrochemical signals and were conjugated with a secondary anti-phosphoserine antibody in a heterogeneous sandwich immunoassay. In this assay, the model phosphorylated protein BSA-OP was added to the primary BSA antibody coated polystyrene microwells, and then the QD labeled anti-phosphoserine antibody was added for completing immunorecognition. Finally, the bound QD was dissolved in an acid-dissolution step and was detected by electrochemical stripping analysis. The measured current responses were proportional to the concentration of BSA-OP. Under optimal conditions, the voltammetric response was linear over the range of 0.5–500
ng
mL
−1 of BSA-OP, with a detection limit of 0.5
ng
mL
−1. It also shows good reproducibility with a relative standard deviation of 8.6%. This QD-based electrochemical immunoassay offers great promise for simple and cost-effective analysis of protein biomarkers.
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Details
- Title
- CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin
- Creators
- Kulwadee Pinwattana - Department of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, ThailandJun Wang - Pacific Northwest National Laboratory, 902 Battele Boulevard Richland, WA 99352, United StatesChiann-Tso Lin - Pacific Northwest National Laboratory, 902 Battele Boulevard Richland, WA 99352, United StatesHong Wu - Pacific Northwest National Laboratory, 902 Battele Boulevard Richland, WA 99352, United StatesDan Du - Pacific Northwest National Laboratory, 902 Battele Boulevard Richland, WA 99352, United StatesYuehe Lin - Pacific Northwest National Laboratory, 902 Battele Boulevard Richland, WA 99352, United StatesOrawon Chailapakul - Department of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand
- Publication Details
- Biosensors & bioelectronics, Vol.26(3), pp.1109-1113
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547242901842
- Language
- English
- Resource Type
- Journal article