Journal article
CdTe@SiO2 signal reporters-based fluorescent immunosensor for quantitative detection of prostate specific antigen
Analytica chimica acta, Vol.1057, pp.44-50
05/30/2019
PMID: 30832917
Abstract
In this paper, an immunosensor using CdTe@SiO2 core-shell nanoparticles as labels was constructed for highly sensitive detection of prostate-specific antigen (PSA). In this approach, CdTe@SiO2 core-shell nanoparticles were synthesized using the sol-gel method. The additional Cd ions and sulfur source in SiO2 shell can greatly enhance the fluorescence intensity of CdTe nanocrystals. The reason is the formation of CdS-like cluster in SiO2 shell, which reduced the quantum size effect. The obtained CdTe@SiO2 nanoparticles also exhibited excellent biocompatibility, which was ideal for applying in biomarker detection. Furthermore, PSA capture antibodies functionalized magnetic Fe3O4 nanoparticles (Fe3O4-Ab1) were utilized in the proposed immunosensor to capture and enrich the PSA. The captured PSA was then immuno-recognized by CdTe@SiO2 labeled with PSA detection antibodies (CdTe@SiO2-Ab(2)) by forming the sandwich complex Fe3O4-Ab(1)/PSA/Ab(2)-CdTe@SiO2. The construction of this immunosensor was confirmed by fluorescence spectroscopy. The proposed immunosensor showed a good linear relationship between the fluorescent intensity and the target PSA concentration ranging from 0.01 to 5 ng/mL, and a detection limit as low as 0.003 ng/mL was achieved. The sensor also exhibited good specificity to PSA. This highly sensitive and specific immunosensor has great potential to be used in other biological detection. (C) 2019 Elsevier B.V. All rights reserved.
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Details
- Title
- CdTe@SiO2 signal reporters-based fluorescent immunosensor for quantitative detection of prostate specific antigen
- Creators
- Yuting Zhao - Washington State UniversityWen Gao - Shenzhen UniversityXiaoxiao Ge - Washington State UniversitySuiqiong Li - Washington State UniversityDan Du - Washington State UniversityHaipeng Yang - Shenzhen University
- Publication Details
- Analytica chimica acta, Vol.1057, pp.44-50
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- 201886078 / Shenzhen Science and Technology Research grant
- Identifiers
- 99901227845501842
- Language
- English
- Resource Type
- Journal article