Journal article
Cell Cycle Dysregulation by Human Cytomegalovirus: Influence of the Cell Cycle Phase at the Time of Infection and Effects on Cyclin Transcription
Journal of virology, Vol.72(5), pp.3729-3741
05/1998
Handle:
https://hdl.handle.net/2376/105715
PMCID: PMC109595
PMID: 9557655
Abstract
Human cytomegalovirus (HCMV) infection inhibits cell cycle progression and alters the expression of cyclins E, A, and B (F. M. Jault, J.-M. Jault, F. Ruchti, E. A. Fortunato, C. Clark, J. Corbeil, D. D. Richman, and D. H. Spector, J. Virol. 69:6697–6704, 1995). In this study, we examined cell cycle progression, cyclin gene expression, and early viral events when the infection was initiated at different points in the cell cycle (G
0
, G
1
, and S). In all cases, infection led to cell cycle arrest. Cells infected in G
0
or G
1
phase also showed a complete or partial absence, respectively, of cellular DNA synthesis at a time when DNA synthesis occurred in the corresponding mock-infected cells. In contrast, when cells were infected near or during S phase, many cells were able to pass through S phase and undergo mitosis prior to cell cycle arrest. S-phase infection also produced a delay in the appearance of the viral cytopathic effect and in the synthesis of immediate-early and early proteins. Labeling of cells with bromodeoxyuridine immediately prior to HCMV infection in S phase revealed that viral protein expression occurred primarily in cells which were not engaged in DNA synthesis at the time of infection. The viral-mediated induction of cyclin E, maintenance of cyclin-B protein levels, and inhibitory effects on the accumulation of cyclin A were not significantly affected when infection occurred during different phases of the cell cycle (G
0
, G
1
, and S). However, there was a delay in the observed inhibition of cyclin A in cells infected during S phase. This finding was in accord with the pattern of cell cycle progression and delay in viral gene expression associated with S-phase infection. Analysis of the mRNA revealed that the effects of the virus on cyclin E and cyclin A, but not on cyclin B, were primarily at the transcriptional level.
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Details
- Title
- Cell Cycle Dysregulation by Human Cytomegalovirus: Influence of the Cell Cycle Phase at the Time of Infection and Effects on Cyclin Transcription
- Creators
- Bryan S Salvant - Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0357Elizabeth A Fortunato - Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0357Deborah H Spector - Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0357
- Publication Details
- Journal of virology, Vol.72(5), pp.3729-3741
- Academic Unit
- UNKNOWN
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547086001842
- Language
- English
- Resource Type
- Journal article