Journal article
Cell-surface expression of PrPC and the presence of scrapie prions in the blood of goats
Journal of general virology, Vol.93(Pt 5), pp.1127-1131
05/2012
Handle:
https://hdl.handle.net/2376/104496
PMID: 22278824
Abstract
Although host-encoded prion protein (PrP(C)) expression in ovine PBMCs and prion infectivity in scrapie-infected sheep blood have been demonstrated, such studies have not been reported in goats. Therefore, this study characterized cell-surface expression of PrP(C) on PBMC subsets derived from normal goats and sheep, by flow cytometry, and determined prion infectivity in blood from a scrapie-infected goat using a transfusion bioassay in goat kids. Cell-surface PrP(C) expression was detected on all subsets of goat PBMCs. The highest PrP(C) cell-surface expression was found in CD2(+) T lymphocytes in goats. Transmission of infection was detected in all three recipients who received whole blood from a goat with classical scrapie. It was concluded that caprine PBMCs express PrP(C) similarly to sheep but with relative differences among PBMCs subsets, and that blood-borne infectious prions can be detected in scrapie-infected goats. Thus, similar to sheep, goat blood may be a suitable diagnostic target for the detection of scrapie infection.
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Details
- Title
- Cell-surface expression of PrPC and the presence of scrapie prions in the blood of goats
- Creators
- Rohana P Dassanayake - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USADavid A Schneider - Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-6630, USALynn M Herrmann-Hoesing - Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-6630, USAThomas C Truscott - Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-6630, USAWilliam C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAKatherine I O'Rourke - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA
- Publication Details
- Journal of general virology, Vol.93(Pt 5), pp.1127-1131
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- England
- Identifiers
- 99900546652401842
- Language
- English
- Resource Type
- Journal article