Journal article
Characterisation of a monoclonal antibody recognising the CD3 ε chain of the bovine T cell receptor complex
Veterinary immunology and immunopathology, Vol.61(1), pp.25-35
1998
Handle:
https://hdl.handle.net/2376/113896
PMID: 9613470
Abstract
We describe the characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine
α
β and
γ
δTcR
+ T cells. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3
ε chain, reacted with mAb MM1A indicating that the epitope recognised is on the
ε chain of the complex and confirming that the mAb recognised bovine CD3.
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Details
- Title
- Characterisation of a monoclonal antibody recognising the CD3 ε chain of the bovine T cell receptor complex
- Creators
- N.D MacHugh - International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi, KenyaJ.K Mburu - International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi, KenyaM.J Hamilton - Washington State University, Pullman, WA, USAW.C Davis - Washington State University, Pullman, WA, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.61(1), pp.25-35
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548469201842
- Language
- English
- Resource Type
- Journal article