Characterization and expression of steroidogenic acute regulatory protein and MLN64 cDNAs in trout

Makoto Kusakabe; Takashi Todo; H James McQuillan; Frederick W Goetz; Graham Young

doi:10.1210/en.143.6.2062

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Characterization and expression of steroidogenic acute regulatory protein and MLN64 cDNAs in trout

Journal article

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Peer reviewed

Characterization and expression of steroidogenic acute regulatory protein and MLN64 cDNAs in trout

Makoto Kusakabe, Takashi Todo, H James McQuillan, Frederick W Goetz and Graham Young

Endocrinology (Philadelphia), Vol.143(6), pp.2062-2070

06/2002

DOI: https://doi.org/10.1210/en.143.6.2062

Handle:

https://hdl.handle.net/2376/114278

PMID: 12021170

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Abstract

DNA, Complementary - genetics

Molecular Sequence Data

Tissue Distribution

RNA, Messenger - biosynthesis

Kidney - metabolism

In Situ Hybridization

Stress, Physiological - metabolism

Cloning, Molecular

DNA, Complementary - biosynthesis

Female

Ovarian Follicle - metabolism

Carrier Proteins

Amino Acid Sequence

Phosphoproteins - biosynthesis

Oncorhynchus mykiss - genetics

Phosphoproteins - genetics

Reverse Transcriptase Polymerase Chain Reaction

DNA, Complementary - isolation & purification

Blotting, Northern

Membrane Proteins - biosynthesis

Animals

Oocytes - physiology

Transcription, Genetic - genetics

In Vitro Techniques

Ovulation - physiology

Complementary DNA-encoding proteins with high homology to steroidogenic acute regulatory proteins (StAR) of mammals were cloned from rainbow trout head kidney and a mixture of several brook trout tissues. A cDNA encoding an MLN64 homolog was also cloned from brook trout. The C-terminal domains of rainbow trout StAR and brook trout StAR were very highly conserved compared with StAR of mammals. In rainbow trout, Northern and RT-PCR analyses showed abundant StAR transcripts in head kidney, ovary, and testis, and weaker signals were found in intestine, pyloric caeca, spleen, and kidney. Brief acute stress resulted in elevated plasma cortisol levels and a 2-fold increase in rainbow trout StAR transcripts in head kidneys sampled 3 h after exposure to the stressor. In brook trout, StAR transcripts were detected only in known steroidogenic tissues. Ovarian brook trout StAR mRNA was not seen until the onset of final maturation. Its abundance increased during germinal vesicle breakdown, peaked during and just following ovulation, and decreased by 2 wk post ovulation. Brook trout MLN64 transcripts were found in all tissues tested, and transcript abundance in ovarian samples did not vary during final oocyte maturation and ovulation. Both StAR structure and function appear to be highly conserved throughout the vertebrates.

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Title: Characterization and expression of steroidogenic acute regulatory protein and MLN64 cDNAs in trout
Creators: Makoto Kusakabe - Department of Zoology, University of Otago, Dunedin 9001, New Zealand
Takashi Todo
H James McQuillan
Frederick W Goetz
Graham Young
Publication Details: Endocrinology (Philadelphia), Vol.143(6), pp.2062-2070
Academic Unit: UNKNOWN
Publisher: United States
Identifiers: 99900547402701842
Language: English
Resource Type: Journal article