Journal article
Characterization of 463 Type I markers suitable for dog genome mapping
Mammalian genome, Vol.10(8), pp.803-813
08/1999
Handle:
https://hdl.handle.net/2376/114455
PMID: 10430667
Abstract
In total, 463 canine gene markers were identified and characterized to serve as reagents in canine genome map projects. These markers are distributed over 221 canine gene markers, 139 TOASTs (Traced Orthologous Sequence Tags), 27 canine TOASTs, and 76 huESTs (human Expressed Sequence Tags). Out of 310 canine gene markers, 59%–84% were successfully amplified on dog DNA, the highest rates of success being observed when the exon/intron structure is known. Concerning TOASTs and human ESTs, of the 225 and 300 markers analyzed, 62% and 25% respectively were able to produce a dog positive amplification. As part of an ongoing project to map the canine genome using a dog/hamster radiation hybrid panel, these markers were tested for their specificity on dog versus hamster DNA. Thus 61%, 21%, and 12% of dog gene markers, TOASTs, and huESTs met the criteria required for radiation hybrid mapping, respectively. All of these 463 canine gene markers, however, are available and will be of value to any other mapping strategies.
Metrics
7 Record Views
Details
- Title
- Characterization of 463 Type I markers suitable for dog genome mapping
- Creators
- Catherine Priat - Laboratoire de Biochimie et Biologie Moléculaire, UPR41CNRS ``Recombinaisons Génétiques'', Faculté de Médecine, 2 avenue du Professeur Léon Bernard, 35043 Rennes cedex, France FRZhihua H Jiang - Laboratoire de Biochimie et Biologie Moléculaire, UPR41CNRS ``Recombinaisons Génétiques'', Faculté de Médecine, 2 avenue du Professeur Léon Bernard, 35043 Rennes cedex, France FRCorinna Renier - Laboratoire de Biochimie et Biologie Moléculaire, UPR41CNRS ``Recombinaisons Génétiques'', Faculté de Médecine, 2 avenue du Professeur Léon Bernard, 35043 Rennes cedex, France FRCatherine André - Laboratoire de Biochimie et Biologie Moléculaire, UPR41CNRS ``Recombinaisons Génétiques'', Faculté de Médecine, 2 avenue du Professeur Léon Bernard, 35043 Rennes cedex, France FRFrancis Galibert - Laboratoire de Biochimie et Biologie Moléculaire, UPR41CNRS ``Recombinaisons Génétiques'', Faculté de Médecine, 2 avenue du Professeur Léon Bernard, 35043 Rennes cedex, France FR
- Publication Details
- Mammalian genome, Vol.10(8), pp.803-813
- Academic Unit
- Animal Sciences, Department of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900547865601842
- Language
- English
- Resource Type
- Journal article