Journal article
Characterization of Biosynthetic Pathways for the Production of the Volatile Homoterpenes DMNT and TMTT in Zea mays
The Plant cell, Vol.28(10), pp.2651-2665
10/2016
Handle:
https://hdl.handle.net/2376/116437
PMCID: PMC5134970
PMID: 27662898
Abstract
Plant volatiles not only have multiple defense functions against herbivores, fungi, and bacteria, but also have been implicated in signaling within the plant and toward other organisms. Elucidating the function of individual plant volatiles will require more knowledge of their biosynthesis and regulation in response to external stimuli. By exploiting the variation of herbivore-induced volatiles among 26 maize (Zea mays) inbred lines, we conducted a nested association mapping and genome-wide association study (GWAS) to identify a set of quantitative trait loci (QTLs) for investigating the pathways of volatile terpene production. The most significant identified QTL affects the emission of (E)-nerolidol, linalool, and the two homoterpenes (E)-3,8-dimethyl-1,4,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT). GWAS associated a single nucleotide polymorphism in the promoter of the gene encoding the terpene synthase TPS2 with this QTL Biochemical characterization of TPS2 verified that this plastid-localized enzyme forms linalool, (E)-nerolidol, and (E,E)-geranyllinalool. The subsequent conversion of (E)-nerolidol into DMNT maps to a P450 monooxygenase, CYP92C5, which is capable of converting nerolidol into DMNT by oxidative degradation. A QTL influencing TMTT accumulation corresponds to a similar monooxygenase, CYP92C6, which is specific for the conversion of (E,E)-geranyllinalool to TMTT The DMNT biosynthetic pathway and both monooxygenases are distinct from those previously characterized for DMNT and TMTT synthesis in Arabidopsis thaliana, suggesting independent evolution of these enzymatic activities.
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Details
- Title
- Characterization of Biosynthetic Pathways for the Production of the Volatile Homoterpenes DMNT and TMTT in Zea mays
- Creators
- Annett Richter - Institute for Pharmacy, Martin Luther University Halle-Wittenberg, D-06120 Halle, GermanyClaudia Schaff - Institute for Pharmacy, Martin Luther University Halle-Wittenberg, D-06120 Halle, GermanyZhiwu Zhang - Institute for Genomic Diversity, Cornell University, Ithaca, New York 14853Alexander E Lipka - Institute for Genomic Diversity, Cornell University, Ithaca, New York 14853Feng Tian - Institute for Genomic Diversity, Cornell University, Ithaca, New York 14853Tobias G Köllner - Max Planck Institute for Chemical Ecology, D-07745 Jena, GermanyChristiane Schnee - Max Planck Institute for Chemical Ecology, D-07745 Jena, GermanySusanne Preiß - Institute for Pharmacy, Martin Luther University Halle-Wittenberg, D-06120 Halle, GermanySandra Irmisch - Max Planck Institute for Chemical Ecology, D-07745 Jena, GermanyGeorg Jander - Boyce Thompson Institute for Plant Research, Ithaca, New York 14853Willhelm Boland - Max Planck Institute for Chemical Ecology, D-07745 Jena, GermanyJonathan Gershenzon - Max Planck Institute for Chemical Ecology, D-07745 Jena, GermanyEdward S Buckler - U.S. Department of Agriculture-Agricultural Research Service, Ithaca, New York 14853Jörg Degenhardt - Institute for Pharmacy, Martin Luther University Halle-Wittenberg, D-06120 Halle, Germany joerg.degenhardt@pharmazie.uni-halle.de
- Publication Details
- The Plant cell, Vol.28(10), pp.2651-2665
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- United States
- Identifiers
- 99900548207301842
- Language
- English
- Resource Type
- Journal article