Journal article
Characterization of Class III Peroxidases from Switchgrass
Plant physiology (Bethesda), Vol.173(1), pp.417-433
01/2017
Handle:
https://hdl.handle.net/2376/106834
PMID: 27879392
Abstract
Class III peroxidases (CIIIPRX) catalyze the oxidation of monolignols, generate radicals, and ultimately lead to the formation of lignin. In general, CIIIPRX genes encode a large number of isozymes with ranges of in vitro substrate specificities. In order to elucidate the mode of substrate specificity of these enzymes, we characterized one of the CIIIPRXs (PviPRX9) from switchgrass (Panicum virgatum), a strategic plant for second-generation biofuels. The crystal structure, kinetic experiments, molecular docking, as well as expression patterns of PviPRX9 across multiple tissues and treatments, along with its levels of coexpression with the majority of genes in the monolignol biosynthesis pathway, revealed the function of PviPRX9 in lignification. Significantly, our study suggested that PviPRX9 has the ability to oxidize a broad range of phenylpropanoids with rather similar efficiencies, which reflects its role in the fortification of cell walls during normal growth and root development and in response to insect feeding. Based on the observed interactions of phenylpropanoids in the active site and analysis of kinetics, a catalytic mechanism involving two water molecules and residues histidine-42, arginine-38, and serine-71 was proposed. In addition, proline-138 and gluntamine-140 at the
P-X-P-X
motif, leucine-66, proline-67, and asparagine-176 may account for the broad substrate specificity of PviPRX9. Taken together, these observations shed new light on the function and catalysis of PviPRX9 and potentially benefit efforts to improve biomass conservation properties in bioenergy and forage crops.
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Details
- Title
- Characterization of Class III Peroxidases from Switchgrass
- Creators
- Timothy W Moural - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)Kevin M Lewis - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)Carlo Barnaba - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)Fang Zhu - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)Nathan A Palmer - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)Gautam Sarath - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)Erin D Scully - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)Jeffrey P Jones - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)Scott E Sattler - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.)ChulHee Kang - Stored Product Insect and Engineering Research Unit, United States Department of Agriculture-Agricultural Research Service Center for Grain and Animal Health, Manhattan, Kansas 66502 (E.D.S.) chkang@wsu.edu
- Publication Details
- Plant physiology (Bethesda), Vol.173(1), pp.417-433
- Academic Unit
- Chemistry, Department of
- Publisher
- United States
- Grant note
- R01 GM111254 / NIGMS NIH HHS
- Identifiers
- 99900547000501842
- Language
- English
- Resource Type
- Journal article