Journal article
Characterization of Mono- and Mixed-Culture Campylobacter jejuni Biofilms
Applied and environmental microbiology, Vol.78(4), pp.1033-1038
02/2012
Handle:
https://hdl.handle.net/2376/115641
PMCID: PMC3273011
PMID: 22179238
Abstract
Campylobacter jejuni
, one of the most common causes of human gastroenteritis, is a thermophilic and microaerophilic bacterium. These characteristics make it a fastidious organism, which limits its ability to survive outside animal hosts. Nevertheless,
C. jejuni
can be transmitted to both humans and animals via environmental pathways, especially through contaminated water. Biofilms may play a crucial role in the survival of the bacterium under unfavorable environmental conditions. The goal of this study was to investigate survival strategies of
C. jejuni
in mono- and mixed-culture biofilms. We grew monoculture biofilms of
C. jejuni
and mixed-culture biofilms of
C. jejuni
with
Pseudomonas aeruginosa
. We found that mono- and mixed-culture biofilms had significantly different structures and activities. Monoculture
C. jejuni
biofilms did not consume a measurable quantity of oxygen. Using a confocal laser scanning microscope (CLSM), we found that cells from monoculture biofilms were alive according to live/dead staining but that these cells were not culturable. In contrast, in mixed-culture biofilms,
C. jejuni
remained in a culturable physiological state. Monoculture
C. jejuni
biofilms could persist under lower flow rates (0.75 ml/min) but were unable to persist at higher flow rates (1 to 2.5 ml/min). In sharp contrast, mixed-culture biofilms were more robust and were unaffected by higher flow rates (2.5 ml/min). Our results indicate that biofilms provide an environmental refuge that is conducive to the survival of
C. jejuni
.
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Details
- Title
- Characterization of Mono- and Mixed-Culture Campylobacter jejuni Biofilms
- Creators
- Tuba Ica - Dumlupinar University, Faculty of Art and Science, Department of Biology, Kutahya, TurkeyVildan Caner - Pamukkale University, Faculty of Medicine, Department of Medical Biology, Denizli, TurkeyOzlem Istanbullu - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USAHung Duc Nguyen - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USABulbul Ahmed - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USADouglas R Call - Department of Veterinary Microbiology and Pathology and the Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USAHaluk Beyenal - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USA
- Publication Details
- Applied and environmental microbiology, Vol.78(4), pp.1033-1038
- Academic Unit
- Paul G. Allen School for Global Animal Health; Chemical Engineering and Bioengineering, School of
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900547783001842
- Language
- English
- Resource Type
- Journal article