Journal article
Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D
Biochemical journal, Vol.314(1), pp.15-19
02/15/1996
Handle:
https://hdl.handle.net/2376/114353
PMCID: PMC1217018
PMID: 8660276
Abstract
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.
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Details
- Title
- Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D
- Creators
- Krishna M ELLA - Departments of Cell and Molecular Pharmacology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2251, U.S.AJoseph W DOLAN - Departments of Microbiology/Immunology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2251, U.S.AChen QI - Departments of Cell and Molecular Pharmacology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2251, U.S.AKathryn E MEIER - Departments of Cell and Molecular Pharmacology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2251, U.S.A
- Publication Details
- Biochemical journal, Vol.314(1), pp.15-19
- Academic Unit
- Pharmaceutical Sciences, Department of
- Identifiers
- 99900548474901842
- Language
- English
- Resource Type
- Journal article