Characterization of WC1 co-receptors on functionally distinct subpopulations of ruminant gamma delta T cells

Aric N Rogers; Denille G Vanburen; Baixiang Zou; Kevin K Lahmers; Carolyn T A Herzig; Wendy C Brown; Janice C Telfer; Cynthia L Baldwin

doi:10.1016/j.cellimm.2006.05.006

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Characterization of WC1 co-receptors on functionally distinct subpopulations of ruminant gamma delta T cells

Journal article

Peer reviewed

Characterization of WC1 co-receptors on functionally distinct subpopulations of ruminant gamma delta T cells

Aric N Rogers, Denille G Vanburen, Baixiang Zou, Kevin K Lahmers, Carolyn T A Herzig, Wendy C Brown, Janice C Telfer and Cynthia L Baldwin

Cellular immunology, Vol.239(2), pp.151-161

02/2006

DOI: https://doi.org/10.1016/j.cellimm.2006.05.006

Handle:

https://hdl.handle.net/2376/109813

PMID: 16828467

Abstract

T-Lymphocyte Subsets - immunology

Amino Acid Sequence

Receptors, Antigen, T-Cell, gamma-delta - chemistry

Receptors, Antigen, T-Cell, gamma-delta - metabolism

Membrane Glycoproteins - chemistry

Cells, Cultured

Molecular Sequence Data

Cytoplasm - metabolism

Membrane Glycoproteins - genetics

Animals

Cytoplasm - immunology

Cattle

T-Lymphocyte Subsets - metabolism

Cell Membrane - immunology

Cell Membrane - metabolism

Clone Cells

WC1 molecules are implicated in augmenting cellular activation as well as inducing cell cycle arrest of gammadelta T cells. Since WC1 is a large multigene family differences in outcome could result from modulation of different WC1 molecules. To further investigate this family of molecules, peripheral blood WC1(+) gammadelta T cell subpopulations were evaluated by 2-D Western blotting and RT-PCR. We found 13 cDNA intracytoplasmic tail sequences with differences in signaling motifs among them and at least 20 biochemically distinguishable WC1 spots associated with cell membranes, with some in lipid rafts. An understanding of the diversity of 2-D spots could not be resolved by evaluating T cell clones, removing sialyated carbohydrates or blotting with anti-WC1.1 or anti-WC1.2-specific antibodies. Nevertheless, while the major gammadelta T cell subpopulations in blood (WC1.1(+)/WC1.2(-) and WC1.2(+)/WC1.1(-)) both had complex 2-D patterns, virtually all spots associated with WC1.2(+)/WC1.1(-) cells bore the WC1.2 epitope, distinguishing them from the WC1.1(+) cells.

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Title: Characterization of WC1 co-receptors on functionally distinct subpopulations of ruminant gamma delta T cells
Creators: Aric N Rogers - Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA
Denille G Vanburen
Baixiang Zou
Kevin K Lahmers
Carolyn T A Herzig
Wendy C Brown
Janice C Telfer
Cynthia L Baldwin
Publication Details: Cellular immunology, Vol.239(2), pp.151-161
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: Netherlands
Grant note: AI44005 / NIAID NIH HHS K08 AI53594 / NIAID NIH HHS
Identifiers: 99900547052001842
Language: English
Resource Type: Journal article

Characterization of WC1 co-receptors on functionally distinct subpopulations of ruminant gamma delta T cells

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