Journal article
Characterization of a Novel Transcript of 14–3‐3 Theta in Sertoli Cells
Journal of andrology, Vol.21(5), pp.730-738
09/2000
Handle:
https://hdl.handle.net/2376/111666
PMID: 10975420
Abstract
The isoforms of the highly conserved and ubiquitously expressed 14‐3‐3 family of proteins function primarily as adapters that modulate interactions between components of various cellular signaling and cell cycle regulatory pathways. Low levels of 14‐3‐3 isoforms appear to be expressed in most tissues, but specific isoforms or combinations have been shown to be overexpressed in a cell‐specific manner. In the present study we show that the theta isoform of 14‐3‐3 is expressed in Sertoli cells. Although previous reports have shown the presence of a 14‐3‐3 theta isoform in mouse testicular germ cells, this report demonstrates the presence of the 14‐3‐3 theta isoform in rat Sertoli cells. The 14‐3‐3 theta isoform isolated from rat Sertoli cells appears to have a truncated 3′ UTR, which makes the transcript shorter by 244 bp, compared with its brain counterpart. Northern blot analysis suggests that the 14‐3‐3 theta isoform may also be present in other testicular cell types and tissues. The truncation of the 3′ UTR suggests a potential role in regulating cell‐specific expression of 14‐3‐3 theta. The expression of 14‐3‐3 theta in Sertoli cells was confirmed by Northern blot, polymerase chain reaction, Western blot, and immunocytochemical analysis. The levels of 14‐3‐3 theta mRNA and protein in Sertoli cells remained unchanged in response to the gonadotropin, FSH. Consistent with the absence of the effect of FSH on the expression of 14‐3‐3 theta, an antisense oligonucleotide to 14‐3‐3 theta had no effect on FSH‐induced activation of the transferrin promoter in Sertoli cells. The widespread expression of 14‐3‐3 theta in testis and the lack of effect of FSH on levels of its expression suggest that 14‐3‐3 theta influences Sertoli cell function in an FSH‐independent manner.
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Details
- Title
- Characterization of a Novel Transcript of 14–3‐3 Theta in Sertoli Cells
- Creators
- JAIDEEP CHAUDHARYMICHAEL K SKINNER
- Publication Details
- Journal of andrology, Vol.21(5), pp.730-738
- Academic Unit
- Biological Sciences, School of
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- Number of pages
- 9
- Identifiers
- 99900547420301842
- Language
- English
- Resource Type
- Journal article