Journal article
Characterization of a Shiga Toxin-, Intimin-, and Enterotoxin Hemolysin-Producing Escherichia coli ONT:H25 Strain Commonly Isolated from Healthy Cattle
Journal of clinical microbiology, Vol.43(7), pp.3213-3220
07/2005
Handle:
https://hdl.handle.net/2376/114513
PMCID: PMC1169089
PMID: 16000438
Abstract
Among bovine fecal and recto-anal mucosal swab samples cultured in our laboratory for
Escherichia coli
O157:H7, we frequently isolated
E. coli
organisms that were phenotypically similar to the O157:H7 serotype as non-sorbitol fermenting and negative for β-glucuronidase activity but serotyped O nontypeable:H25 (ONT:H25). This study determined the prevalence and virulence properties of the
E. coli
ONT:H25 isolates. Among dairy and feedlot cattle (
n
= 170) sampled in Washington, Idaho, and Alberta, Canada, the percentage of animals culture positive for
E. coli
ONT:H25 ranged from 7.5% to 22.5%, compared to the prevalence of
E. coli
O157:H7 that ranged from 0% to 15%. A longitudinal 8-month study of dairy heifers (
n
= 40) showed that 0 to 15% of the heifers were culture positive for
E. coli
O157:H7, while 15 to 22.5% of the animals were culture positive for
E. coli
ONT:H25. As determined by a multiplex PCR, the
E. coli
ONT:H25 isolates carried a combination of virulence genes characteristic of the enterohemorrhagic
E. coli
, including intimin, translocated intimin receptor, Stx2, and hemolysin (
eae-
β,
tir
,
stx
2vh-a
, and
hly
).
E. coli
ONT:H25 isolates from diverse geographic locations and over time were fingerprinted by separating XbaI-restricted chromosomal DNA by pulsed-field gel electrophoresis (PFGE) separation. Two strains of
E. coli
ONT:H25 were highly similar by PFGE pattern. Experimental inoculation of cattle showed that
E. coli
ONT:H25, like
E. coli
O157:H7, colonized the bovine recto-anal junction mucosa for more than 4 weeks following a single rectal application of bacteria.
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Details
- Title
- Characterization of a Shiga Toxin-, Intimin-, and Enterotoxin Hemolysin-Producing Escherichia coli ONT:H25 Strain Commonly Isolated from Healthy Cattle
- Creators
- Haiqing Sheng - University of Idaho, Department of Microbiology, Molecular Biology, and Biochemistry, Moscow, Idaho 83844-3052Margaret A Davis - University of Idaho, Department of Microbiology, Molecular Biology, and Biochemistry, Moscow, Idaho 83844-3052Hannah J Knecht - University of Idaho, Department of Microbiology, Molecular Biology, and Biochemistry, Moscow, Idaho 83844-3052Dale D Hancock - University of Idaho, Department of Microbiology, Molecular Biology, and Biochemistry, Moscow, Idaho 83844-3052Joyce Van Donkersgoed - University of Idaho, Department of Microbiology, Molecular Biology, and Biochemistry, Moscow, Idaho 83844-3052Carolyn J Hovde - University of Idaho, Department of Microbiology, Molecular Biology, and Biochemistry, Moscow, Idaho 83844-3052
- Publication Details
- Journal of clinical microbiology, Vol.43(7), pp.3213-3220
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547577001842
- Language
- English
- Resource Type
- Journal article