Journal article
Characterization of a functional angiotensin IV receptor on coronary microvascular endothelial cells
Regulatory peptides, Vol.58(3), pp.107-115
1995
Handle:
https://hdl.handle.net/2376/107851
PMID: 8577922
Abstract
A new class of angiotensin receptors has recently been identified that exhibits both high specificity and affinity for the hexapeptide (3–8) fragment of angiotensin II, angiotensin IV (AngIV). Here, utilizing radioligand binding, we fully characterize AngIV binding at the AT4 receptor on cultured bovine coronary venular endothelial cells (CVEC), and report that when AngIV and bFGF are presented simultaneously an enhancement of DNA synthesis results that is significantly greater than that produced by bFGF alone. The level of DNA synthesis was determined by the incorporation of [
3H]thymidine into quiescent CVEC monolayers following exposure to 10 nM AngIV and 10 ng/ml bFGF for 1, 3, 5, 7, 9, or 11 days. A significant enhancement of DNA synthesis (
P < 0.01) was seen following 3, 5, 7, 9 and 11 days exposure. In addition, AngIV does not bind to bFGF or heparin, and conversely, bFGF is unable to compete for AngIV binding which suggests that this synergistic response is mediated by independent receptors for these ligands. Results of this study indicate that microvascular endothelial cells are significantly more responsive to bFGF in the presence of nanomolar concentrations of AngIV.
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Details
- Title
- Characterization of a functional angiotensin IV receptor on coronary microvascular endothelial cells
- Creators
- Keith L Hall - Department of Biology, San Diego State University, 5300 Campanile Dr., San Diego, CA 92182, USASubramaniam Venkateswaran - Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, USAJodie M Hanesworth - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA, USAMargaret E Schelling - Department of Genetics and Cell Biology, Washington State University, Pullman, WA, USAJoseph W Harding - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA, USA
- Publication Details
- Regulatory peptides, Vol.58(3), pp.107-115
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547156901842
- Language
- English
- Resource Type
- Journal article