Journal article
Characterization of a major outer membrane protein multigene family in Ehrlichia ruminantium
Gene, Vol.330(1-2), pp.159-168
2004
Handle:
https://hdl.handle.net/2376/112829
PMID: 15087135
Abstract
Ehrlichia ruminantium is a tick-transmitted rickettsial pathogen, which causes heartwater or cowdriosis in wild and domestic ruminants. A dominant antibody response of animals infected with
E. ruminantium is directed against the outer membrane protein MAP1 (major antigenic protein 1). Part of the locus containing
map1 has been characterized and consists of four
map1 paralogs, designated
map1-2,
map1-1,
map1 and
map1+1, indicating that
map1 is encoded by a multigene family. The purpose of this study was to determine the total number of
map1 paralogs and their transcriptional activities. Using genome walking and data from an ongoing
E. ruminantium genome sequencing project at the Onderstepoort Veterinary Institute, we found 16 paralogs of the
map1 gene tandemly arranged in a 25 kb region of the
E. ruminantium genome. The
map1 multigene family is downstream of a hypothetical transcriptional regulator gene and upstream of the
secA gene. Thirteen paralogs at the 5′ end of the 25-kb locus were connected by short intergenic spaces (ranging from 0 to 42 bp) and the remaining three paralogs at the 3′ end were connected by longer intergenic spaces (ranging from 375 to 1612 bp). All 16
map1 paralogs were transcriptionally active in
E. ruminantium grown in endothelial cells and paralogs with short intergenic spaces were co-transcribed with their adjacent genes.
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Details
- Title
- Characterization of a major outer membrane protein multigene family in Ehrlichia ruminantium
- Creators
- Henriette van Heerden - Department of Chemistry and Biochemistry, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006, South AfricaNicola E Collins - Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South AfricaKelly A Brayton - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USACelia Rademeyer - Institute of Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory, 7925 Cape Town, South AfricaBasil A Allsopp - Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa
- Publication Details
- Gene, Vol.330(1-2), pp.159-168
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547780901842
- Language
- English
- Resource Type
- Journal article