Characterization of a tryptophan 2-monooxygenase gene from Puccinia graminis f. sp. tritici involved in auxin biosynthesis and rust pathogenicity

Chuntao Yin; Jeong-Jin Park; David R Gang; Scot H Hulbert

doi:10.1094/MPMI-09-13-0289-FI

Back

Characterization of a tryptophan 2-monooxygenase gene from Puccinia graminis f. sp. tritici involved in auxin biosynthesis and rust pathogenicity

Journal article

Open access

Characterization of a tryptophan 2-monooxygenase gene from Puccinia graminis f. sp. tritici involved in auxin biosynthesis and rust pathogenicity

Chuntao Yin, Jeong-Jin Park, David R Gang and Scot H Hulbert

Molecular plant-microbe interactions, Vol.27(3), pp.227-235

03/2014

DOI: https://doi.org/10.1094/MPMI-09-13-0289-FI

Handle:

https://hdl.handle.net/2376/104902

PMID: 24350783

Files and links (1)

url

https://doi.org/10.1094/MPMI-09-13-0289-FIView

Published (Version of record) Open

Abstract

DNA, Fungal - genetics

Basidiomycota - physiology

Virulence

Plant Roots - genetics

Seedlings - genetics

Seedlings - microbiology

Mixed Function Oxygenases - metabolism

Plant Diseases - microbiology

Indoleacetic Acids - analysis

Plant Stems - genetics

Triticum - metabolism

Basidiomycota - enzymology

Plants, Genetically Modified

Triticum - microbiology

Plant Growth Regulators - analysis

Pseudomonas syringae - physiology

Gene Expression Regulation, Fungal

Gene Expression

Plant Roots - metabolism

Indoleacetic Acids - metabolism

Gene Silencing

Fungal Proteins - genetics

Plant Roots - microbiology

Plant Stems - microbiology

Arabidopsis - metabolism

Arabidopsis - genetics

Host-Pathogen Interactions

Triticum - genetics

Arabidopsis - microbiology

Phenotype

Plant Leaves - genetics

Plant Leaves - metabolism

Plant Stems - metabolism

Basidiomycota - genetics

Basidiomycota - pathogenicity

Mixed Function Oxygenases - genetics

Plant Growth Regulators - metabolism

Plant Leaves - microbiology

Fungal Proteins - metabolism

Seedlings - metabolism

The plant hormone indole-3-acetic acid (IAA) is best known as a regulator of plant growth and development but its production can also affect plant-microbe interactions. Microorganisms, including numerous plant-associated bacteria and several fungi, are also capable of producing IAA. The stem rust fungus Puccinia graminis f. sp. tritici induced wheat plants to accumulate auxin in infected leaf tissue. A gene (Pgt-IaaM) encoding a putative tryptophan 2-monooxygenase, which makes the auxin precursor indole-3-acetamide (IAM), was identified in the P. graminis f. sp. tritici genome and found to be expressed in haustoria cells in infected plant tissue. Transient silencing of the gene in infected wheat plants indicated that it was required for full pathogenicity. Expression of Pgt-IaaM in Arabidopsis caused a typical auxin expression phenotype and promoted susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000.

Metrics

14 Record Views

See more details

Details

Title: Characterization of a tryptophan 2-monooxygenase gene from Puccinia graminis f. sp. tritici involved in auxin biosynthesis and rust pathogenicity
Creators: Chuntao Yin
Jeong-Jin Park
David R Gang
Scot H Hulbert
Publication Details: Molecular plant-microbe interactions, Vol.27(3), pp.227-235
Academic Unit: Plant Pathology, Department of; Biological Chemistry, Institute of
Publisher: United States
Identifiers: 99900546939401842
Language: English
Resource Type: Journal article