Journal article
Characterization of ovine herpesvirus 2-induced malignant catarrhal fever in rabbits
Veterinary microbiology, Vol.150(3-4), pp.270-277
06/02/2011
Handle:
https://hdl.handle.net/2376/105209
PMID: 21419581
Abstract
Malignant catarrhal fever (MCF) is a frequently fatal lymphoproliferative disease syndrome primarily of ruminant species, caused by gammaherpesviruses in the genus Macavirus. Ovine herpesvirus 2 (OvHV-2), carried by sheep, causes sheep-associated MCF worldwide, while Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest, causes wildebeest-associated MCF, mainly in Africa. Diseases in rabbits can be induced by both viruses, which are clinically and pathologically similar; however, recent studies revealed different expression of viral genes associated with latency or lytic replication during clinical disease between the two viruses. In this study, we further characterized experimentally induced MCF in rabbits by nebulization with OvHV-2 from sheep nasal secretions to elucidate the course of viral replication, along with in vivo incorporation of 5-Bromo-2′-Deoxyuridine (BrdU), to evaluate lymphoproliferation. All six rabbits nebulized with OvHV-2 developed MCF between 24 and 29 days post infection. OvHV-2 DNA levels in peripheral blood leukocytes (PBL) remained undetectable during the incubation period and increased dramatically a few days before onset of clinical signs. During the clinical stage, we found that predominantly lytic gene expression was detected in PBL and tissues, and both T and B cells were proliferating. The data showed that the viral gene expression profile and lymphoproliferation in rabbits with OvHV-2 induced MCF were different from that in rabbits with AlHV-1 induced MCF, suggesting that OvHV-2 and AlHV-1 may play a different role in MCF pathogenesis.
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Details
- Title
- Characterization of ovine herpesvirus 2-induced malignant catarrhal fever in rabbits
- Creators
- Hong Li - Animal Disease Research Unit, USDA-Agricultural Research Service, 3003 ADBF, Washington State University, Pullman, WA 99164-6630, USACristina W Cunha - Animal Disease Research Unit, USDA-Agricultural Research Service, 3003 ADBF, Washington State University, Pullman, WA 99164-6630, USAKatherine L Gailbreath - Animal Disease Research Unit, USDA-Agricultural Research Service, 3003 ADBF, Washington State University, Pullman, WA 99164-6630, USADonal O’Toole - Wyoming Veterinary Diagnostic Laboratory, University of Wyoming, Laramie, WY 82071, USAStephen N White - Animal Disease Research Unit, USDA-Agricultural Research Service, 3003 ADBF, Washington State University, Pullman, WA 99164-6630, USAAlain Vanderplasschen - Department of Infectious and Parasitic Diseases, Immunology-Vaccinology (B43b), Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, BelgiumBenjamin Dewals - Department of Infectious and Parasitic Diseases, Immunology-Vaccinology (B43b), Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, BelgiumDonald. P Knowles - Animal Disease Research Unit, USDA-Agricultural Research Service, 3003 ADBF, Washington State University, Pullman, WA 99164-6630, USANaomi S Taus - Animal Disease Research Unit, USDA-Agricultural Research Service, 3003 ADBF, Washington State University, Pullman, WA 99164-6630, USA
- Publication Details
- Veterinary microbiology, Vol.150(3-4), pp.270-277
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546703101842
- Language
- English
- Resource Type
- Journal article