Journal article
Characterization of the gene encoding a 60-kilodalton Babesia bovis merozoite protein with conserved and surface exposed epitopes
Molecular and biochemical parasitology, Vol.46(1), pp.45-52
1991
Handle:
https://hdl.handle.net/2376/116344
PMID: 1712911
Abstract
A clone expressing a surface exposed, conserved epitope of a 60-kDa merozoite polypeptide was identified in a cDNA library constructed from a cloned Mexico strain of
Babesia bovis. Sequencing of the 1.9-kb insert (pBv60) revealed an open reading frame encoding a 65-kDa polypeptide with a signal peptide and a tandemly repeated region. Monoclonal antibody 23/56.156, which binds a surface exposed epitope on the native polypeptide, specifically immunoprecipitated [
35S]methionine-labeled polypeptides ranging from 60-30 kDa from pBv60 directed transcription and translation. Antibodies raised in rabbits against recombinant polypeptide reacted with the live merozoite surface in a polar immunofluorescence pattern, immunoprecipitated the native 60-kDa polypeptide, and were used to deplete the polypeptide by adsorption from a preparation of native [
35S]methionine-labeled merozoite antigen. Restriction enzyme analysis indicated a single gene copy and the absence of introns. Hybridization demonstrated the presence of the gene in Mexico, Australia ‘L’, and Texas strains of
B. bovis, but not in
Babesia bigemina. A slightly different hybridization pattern was present in uncloned Australia ‘L’
B. bovis, indicating sequence diversity in the Bv60 gene among isolates. Cloning and structural analysis of pBv60 provides a source of defined antigen for determining the role of conserved merozoite surface epitopes in protective immunity against babesiosis.
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Details
- Title
- Characterization of the gene encoding a 60-kilodalton Babesia bovis merozoite protein with conserved and surface exposed epitopes
- Creators
- Carlos E SuarezGuy H PalmerDouglas P JasmerStephen A HinesLance E PerrymanTerry F McElwain
- Publication Details
- Molecular and biochemical parasitology, Vol.46(1), pp.45-52
- Academic Unit
- Veterinary Medicine, College of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900547518801842
- Language
- English
- Resource Type
- Journal article