Chemical methods to detect S-nitrosation

Hua Wang; Ming Xian

doi:10.1016/j.cbpa.2010.10.006

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Chemical methods to detect S-nitrosation

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Chemical methods to detect S-nitrosation

Hua Wang and Ming Xian

Current opinion in chemical biology, Vol.15(1), pp.32-37

02/2011

DOI: https://doi.org/10.1016/j.cbpa.2010.10.006

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https://hdl.handle.net/2376/108449

PMCID: PMC3033964

PMID: 21036657

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Abstract

Biotin - chemistry

Phosphines - chemistry

Nitric Oxide - chemistry

Nitrosation

S-Nitrosothiols - chemistry

Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO-dependent signals. However, the detection and quantification of S-nitrosation in biological samples remain a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is on the methods which can be used to directly conjugate the site(s) of S-nitrosation.

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Details

Title: Chemical methods to detect S-nitrosation
Creators: Hua Wang - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Ming Xian
Publication Details: Current opinion in chemical biology, Vol.15(1), pp.32-37
Academic Unit: Department of Chemistry
Publisher: Elsevier; England
Number of pages: 6
Grant note: R01 GM088226 / NIGMS NIH HHS R01 GM088226-01A1 / NIGMS NIH HHS R01GM088226 / NIGMS NIH HHS
Identifiers: 99900547105101842
Language: English
Resource Type: Accepted manuscript