Journal article
Chemical probes for molecular imaging and detection of hydrogen sulfide and reactive sulfur species in biological systems
Chemical Society reviews, Vol.44(14), pp.4596-4618
07/21/2015
Handle:
https://hdl.handle.net/2376/104495
PMCID: PMC4456340
PMID: 25474627
Abstract
Hydrogen sulfide (H2S), a gaseous species produced by both bacteria and higher eukaryotic organisms, including mammalian vertebrates, has attracted attention in recent years for its contributions to human health and disease. H2S has been proposed as a cytoprotectant and gasotransmitter in many tissue types, including mediating vascular tone in blood vessels as well as neuromodulation in the brain. The molecular mechanisms dictating how H2S affects cellular signaling and other physiological events remain insufficiently understood. Furthermore, the involvement of H2S in metal-binding interactions and formation of related RSS such as sulfane sulfur may contribute to other distinct signaling pathways. Owing to its widespread biological roles and unique chemical properties, H2S is an appealing target for chemical biology approaches to elucidate its production, trafficking, and downstream function. In this context, reaction-based fluorescent probes offer a versatile set of screening tools to visualize H2S pools in living systems. Three main strategies used in molecular probe development for H2S detection include azide and nitro group reduction, nucleophilic attack, and CuS precipitation. Each of these approaches exploits the strong nucleophilicity and reducing potency of H2S to achieve selectivity over other biothiols. In addition, a variety of methods have been developed for the detection of other reactive sulfur species (RSS), including sulfite and bisulfite, as well as sulfane sulfur species and related modifications such as S-nitrosothiols. Access to this growing chemical toolbox of new molecular probes for H2S and related RSS sets the stage for applying these developing technologies to probe reactive sulfur biology in living systems.
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Details
- Title
- Chemical probes for molecular imaging and detection of hydrogen sulfide and reactive sulfur species in biological systems
- Creators
- Vivian S Lin - Department of Chemistry, University of California, Berkeley, California, USAWei Chen - Department of Chemistry, Washington State University, Pullman, Washington, USAMing Xian - Department of Chemistry, Washington State University, Pullman, Washington, USAChristopher J Chang - Howard Hughes Medical Institute, University of California, Berkeley, California, USA
- Publication Details
- Chemical Society reviews, Vol.44(14), pp.4596-4618
- Academic Unit
- Chemistry, Department of
- Publisher
- England
- Grant note
- R01 GM079465 / NIGMS NIH HHS R01 HL116571 / NHLBI NIH HHS Howard Hughes Medical Institute GM 79465 / NIGMS NIH HHS R01HL116571 / NHLBI NIH HHS
- Identifiers
- 99900546548701842
- Language
- English
- Resource Type
- Journal article