Journal article
Chimeric plant calcium/calmodulin-dependent protein kinase gene with a neural visinin-like calcium-binding domain
Proceedings of the National Academy of Sciences - PNAS, Vol.92(11), pp.4897-4901
05/23/1995
Handle:
https://hdl.handle.net/2376/111549
PMCID: PMC41814
PMID: 7761420
Abstract
Calcium, a universal second messenger, regulates diverse cellular processes in eukaryotes. Ca2+ and Ca2+/calmodulin-regulated protein phosphorylation play a pivotal role in amplifying and diversifying the action of Ca(2+)-binding domain was cloned and characterized from lily. The cDNA clone contains an open reading frame coding for a protein of 520 amino acids. The predicted structure of CCaMK contains a catalytic domain followed by two regulatory domains, a calmodulin-binding domain and a visinin-like Ca(2+)-binding domain. The amino-terminal region of CCaMK contains all 11 conserved subdomains characteristic of serine/threonine protein kinases. The calmodulin-binding region of CCaMK has high homology (79%) to alpha subunit of mammalian Ca2+/calmodulin-dependent protein kinase. The calmodulin-binding region is fused to a neural visinin-like domain that contains three Ca(2+)-binding EF-hand motifs and a biotin-binding site. The Escherichia coli-expressed protein (approximately 56 kDa) binds calmodulin in a Ca(2+)-dependent manner. Furthermore, 45Ca-binding assays revealed that CCaMK directly binds Ca2+. The CCaMK gene is preferentially expressed in developing anthers. Southern blot analysis revealed that CCaMK is encoded by a single gene. The structural features of the gene suggest that it has multiple regulatory controls and could play a unique role in Ca2+ signaling in plants.
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Details
- Title
- Chimeric plant calcium/calmodulin-dependent protein kinase gene with a neural visinin-like calcium-binding domain
- Creators
- S Patil - Department of Horticulture, Washington State University, Pullman 99164-6414, USAD Takezawa - Department of Horticulture, Washington State University, Pullman 99164-6414, USAB W Poovaiah - Department of Horticulture, Washington State University, Pullman 99164-6414, USA
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.92(11), pp.4897-4901
- Academic Unit
- Horticulture, Department of
- Identifiers
- 99900547632401842
- Language
- English
- Resource Type
- Journal article