Journal article
Chlamydia trachomatis Slc1 is a type III secretion chaperone that enhances the translocation of its invasion effector substrate TARP
Molecular microbiology, Vol.82(1), pp.131-144
10/2011
Handle:
https://hdl.handle.net/2376/107305
PMCID: PMC3214626
PMID: 21883523
Abstract
Bacterial type III secretion system (T3SSs) chaperones pilot substrates to the export apparatus in a secretion-competent state, and are consequently central to the translocation of effectors into target cells.
Chlamydia trachomatis
is a genetically intractable obligate intracellular pathogen that utilizes T3SS effectors to trigger its entry into mammalian cells. The only well-characterized T3SS effector is TARP (translocated actin recruitment protein), but its chaperone is unknown. Here we exploited a known structural signature to screen for putative type III secretion chaperones encoded within the
C. trachomatis
genome. Using bacterial two-hybrid, co-precipitation, cross-linking, and size exclusion chromatography we show that Slc1 (SycE-like chaperone 1; CT043) specifically interacts with a 200 amino acid residue N-terminal region of TARP (TARP
1–200
). Slc1 formed homodimers
in vitro
, as shown in crosslinking and gel filtration experiments. Biochemical analysis of an isolated Slc1-TARP
1–200
complex was consistent with a characteristic 2:1 chaperone-effector stoichiometry. Furthermore, Slc1 was co-immunoprecipitated with TARP from
C. trachomatis
elementary bodies. Also, co-expression of Slc1 specifically enhanced host cell translocation of TARP by a heterologous
Yersinia enterocolitica
T3SS. Taken together, we propose Slc1 as a chaperone of the
C. trachomatis
T3SS effector TARP.
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Details
- Title
- Chlamydia trachomatis Slc1 is a type III secretion chaperone that enhances the translocation of its invasion effector substrate TARP
- Creators
- Amanda J Brinkworth - Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College, London, United Kingdom SW7 2AZDenise S Malcolm - Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College, London, United Kingdom SW7 2AZAntónio T Pedrosa - Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College, London, United Kingdom SW7 2AZKatarzyna Roguska - Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College, London, United Kingdom SW7 2AZSevanna Shahbazian - Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College, London, United Kingdom SW7 2AZJames E Graham - Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, KY 40210 USARichard D Hayward - Institute of Structural and Molecular Biology, University College London & Birkbeck, London, United Kingdom WC1E 6BTRey A Carabeo - Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College, London, United Kingdom SW7 2AZ
- Publication Details
- Molecular microbiology, Vol.82(1), pp.131-144
- Academic Unit
- Molecular Biosciences, School of
- Grant note
- R01 AI065545-05 || AI / National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID
- Identifiers
- 99900547140701842
- Language
- English
- Resource Type
- Journal article