Chlamydomonas IFT172 is encoded by FLA11, interacts with CrEB1, and regulates IFT at the flagellar tip

Lotte B Pedersen; Mark S Miller; Stefan Geimer; Jeffery M Leitch; Joel L Rosenbaum; Douglas G Cole

doi:10.1016/j.cub.2005.01.037

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Chlamydomonas IFT172 is encoded by FLA11, interacts with CrEB1, and regulates IFT at the flagellar tip

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Chlamydomonas IFT172 is encoded by FLA11, interacts with CrEB1, and regulates IFT at the flagellar tip

Lotte B Pedersen, Mark S Miller, Stefan Geimer, Jeffery M Leitch, Joel L Rosenbaum and Douglas G Cole

Current biology, Vol.15(3), pp.262-266

02/08/2005

DOI: https://doi.org/10.1016/j.cub.2005.01.037

Handle:

https://hdl.handle.net/2376/104117

PMID: 15694311

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Abstract

Amino Acid Sequence

Flagella - physiology

Flagella - ultrastructure

Electrophoresis, Polyacrylamide Gel

Molecular Sequence Data

Immunoblotting

Protein Transport - physiology

Intracellular Signaling Peptides and Proteins - metabolism

Microscopy, Electron

Carrier Proteins - genetics

Microtubules - metabolism

Sequence Alignment

Animals

Carrier Proteins - metabolism

Chlamydomonas reinhardtii - genetics

Chlamydomonas reinhardtii - physiology

Microtubules - genetics

Repetitive Sequences, Nucleic Acid

Flagella - genetics

Gene Components

Intracellular Signaling Peptides and Proteins - genetics

The transport of flagellar precursors and removal of turnover products from the flagellar tip is mediated by intraflagellar transport (IFT) , which is essential for both flagellar assembly and maintenance . Large groups of IFT particles are moved from the flagellar base to the tip by kinesin-2, and smaller groups are returned to the base by cytoplasmic dynein 1b. The IFT particles are composed of two protein complexes, A and B, comprising approximately 16-18 polypeptides. How cargo is unloaded from IFT particles, turnover products loaded, and active IFT motors exchanged at the tip is unknown. We previously showed that the Chlamydomonas microtubule end binding protein 1 (CrEB1) localizes to the flagellar tip and is depleted from the tips of the temperature-sensitive (ts) mutant fla11ts . We demonstrate here that FLA11 encodes IFT protein 172, a component of IFT complex B, and show that IFT172 interacts with CrEB1. Because fla11ts cells are defective in IFT particle turnaround at the tip, our results indicate that IFT172 is involved in regulating the transition between anterograde and retrograde IFT at the tip, perhaps by a mechanism involving CrEB1. Therefore, IFT172 is involved in the control of flagellar assembly/disassembly at the tip.

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Title: Chlamydomonas IFT172 is encoded by FLA11, interacts with CrEB1, and regulates IFT at the flagellar tip
Creators: Lotte B Pedersen - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
Mark S Miller
Stefan Geimer
Jeffery M Leitch
Joel L Rosenbaum
Douglas G Cole
Publication Details: Current biology, Vol.15(3), pp.262-266
Academic Unit: Center for Reproductive Biology
Publisher: England
Grant note: GM14642 / NIGMS NIH HHS GM61920 / NIGMS NIH HHS
Identifiers: 99900546550801842
Language: English
Resource Type: Journal article