Journal article
Chlamydomonas IFT70/CrDYF-1 Is a Core Component of IFT Particle Complex B and Is Required for Flagellar Assembly
Molecular biology of the cell, Vol.21(15), pp.2696-2706
08/2010
Handle:
https://hdl.handle.net/2376/112132
PMCID: PMC2912355
PMID: 20534810
Abstract
DYF-1 is a highly conserved protein essential for ciliogenesis in several model organisms. In Caenorhabditis elegans, DYF-1 serves as an essential activator for an anterograde motor OSM-3 of intraflagellar transport (IFT), the ciliogenesis-required motility that mediates the transport of flagellar precursors and removal of turnover products. In zebrafish and Tetrahymena DYF-1 influences the cilia tubulin posttranslational modification and may have more ubiquitous function in ciliogenesis than OSM-3. Here we address how DYF-1 biochemically interacts with the IFT machinery by using the model organism Chlamydomonas reinhardtii, in which the anterograde IFT does not depend on OSM-3. Our results show that this protein is a stoichiometric component of the IFT particle complex B and interacts directly with complex B subunit IFT46. In concurrence with the established IFT protein nomenclature, DYF-1 is also named IFT70 after the apparent size of the protein. IFT70/CrDYF-1 is essential for the function of IFT in building the flagellum because the flagella of IFT70/CrDYF-1–depleted cells were greatly shortened. Together, these results demonstrate that IFT70/CrDYF-1 is a canonical subunit of IFT particle complex B and strongly support the hypothesis that the IFT machinery has species- and tissue-specific variations with functional ramifications.
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Details
- Title
- Chlamydomonas IFT70/CrDYF-1 Is a Core Component of IFT Particle Complex B and Is Required for Flagellar Assembly
- Creators
- Zhen-Chuan Fan - Department of Biology, Texas A&M University, College Station, TX 77843-3258Robert H Behal - Department of Microbiology, Molecular Biology and Biochemistry and the Center for Reproductive Biology, University of Idaho, Moscow, ID 83844-3052; andStefan Geimer - Electron Microscopy Laboratory, Institute for Cell Biology, University of Bayreuth, 95447 Bayreuth, GermanyZhaohui Wang - Department of Biology, Texas A&M University, College Station, TX 77843-3258Shana M Williamson - Department of Biology, Texas A&M University, College Station, TX 77843-3258Haili Zhang - Department of Biology, Texas A&M University, College Station, TX 77843-3258Douglas G Cole - Department of Microbiology, Molecular Biology and Biochemistry and the Center for Reproductive Biology, University of Idaho, Moscow, ID 83844-3052; andHongmin Qin - Department of Biology, Texas A&M University, College Station, TX 77843-3258
- Contributors
- Tim Stearns (Editor)
- Publication Details
- Molecular biology of the cell, Vol.21(15), pp.2696-2706
- Academic Unit
- Center for Reproductive Biology
- Identifiers
- 99900547511601842
- Language
- English
- Resource Type
- Journal article