Journal article
Chlamydomonas IFT88 and Its Mouse Homologue, Polycystic Kidney Disease Gene Tg737, Are Required for Assembly of Cilia and Flagella
The Journal of cell biology, Vol.151(3), pp.709-718
10/30/2000
Handle:
https://hdl.handle.net/2376/115893
PMCID: PMC2185580
PMID: 11062270
Abstract
Intraflagellar transport (IFT) is a rapid movement of multi-subunit protein particles along flagellar microtubules and is required for assembly and maintenance of eukaryotic flagella. We cloned and sequenced a Chlamydomonas cDNA encoding the IFT88 subunit of the IFT particle and identified a Chlamydomonas insertional mutant that is missing this gene. The phenotype of this mutant is normal except for the complete absence of flagella. IFT88 is homologous to mouse and human genes called Tg737. Mice with defects in Tg737 die shortly after birth from polycystic kidney disease. We show that the primary cilia in the kidney of Tg737 mutant mice are shorter than normal. This indicates that IFT is important for primary cilia assembly in mammals. It is likely that primary cilia have an important function in the kidney and that defects in their assembly can lead to polycystic kidney disease.
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Details
- Title
- Chlamydomonas IFT88 and Its Mouse Homologue, Polycystic Kidney Disease Gene Tg737, Are Required for Assembly of Cilia and Flagella
- Creators
- Gregory J Pazour - Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655Bethany L Dickert - Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655Yvonne Vucica - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520E. Scott Seeley - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520Joel L Rosenbaum - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520George B Witman - Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655Douglas G Cole - Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844
- Publication Details
- The Journal of cell biology, Vol.151(3), pp.709-718
- Academic Unit
- Center for Reproductive Biology
- Identifiers
- 99900548096301842
- Language
- English
- Resource Type
- Journal article