Cholecystokinin increases cytosolic calcium in a subpopulation of cultured vagal afferent neurons

Steven M Simasko; Jason Wiens; Adrienne Karpiel; Mihai Covasa; Robert C Ritter

doi:10.1152/ajpregu.00050.2002

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Cholecystokinin increases cytosolic calcium in a subpopulation of cultured vagal afferent neurons

Journal article

Peer reviewed

Cholecystokinin increases cytosolic calcium in a subpopulation of cultured vagal afferent neurons

Steven M Simasko, Jason Wiens, Adrienne Karpiel, Mihai Covasa and Robert C Ritter

American journal of physiology. Regulatory, integrative and comparative physiology, Vol.283(6), pp.R1303-1313

12/2002

DOI: https://doi.org/10.1152/ajpregu.00050.2002

Handle:

https://hdl.handle.net/2376/107368

PMID: 12388458

Abstract

Receptors, Cholecystokinin - antagonists & inhibitors

Calcium - metabolism

Sincalide - pharmacology

Male

Cholecystokinin - antagonists & inhibitors

Nodose Ganglion - cytology

Nodose Ganglion - metabolism

Cholecystokinin - administration & dosage

Dose-Response Relationship, Drug

Intracellular Fluid - drug effects

Time Factors

Nodose Ganglion - drug effects

Fura-2

Intracellular Fluid - metabolism

Neurons, Afferent - drug effects

Cholecystokinin - analogs & derivatives

Sincalide - analogs & derivatives

Cholecystokinin - pharmacology

Rats

Rats, Sprague-Dawley

Receptors, Cholecystokinin - metabolism

Animals

Receptor, Cholecystokinin B

Receptor, Cholecystokinin A

Neurons, Afferent - metabolism

Imaging fluorescent measurements with fura 2 were used to examine cytosolic calcium signals induced by sulfated CCK octapeptide (CCK-8) in dissociated vagal afferent neurons from adult rat nodose ganglia. We found that 40% (184/465) of the neurons responded to CCK-8 with a transient increase in cytosolic calcium. The threshold concentration of CCK-8 for inducing the response varied from 0.01 to 100 nM. In most neurons (13/16) the response was eliminated by removing extracellular calcium. Depleting intracellular calcium stores with thapsigargin slightly augmented the response. Most neurons were unresponsive to nonsulfated CCK-8. The response was eliminated by the CCK-A receptor antagonist lorglumide. Low concentrations of JMV-180 had no effect; however, high concentrations of JMV-180 reduced responses to CCK-8. These results demonstrate that CCK acts at the low-affinity site of the CCK-A receptor to trigger the entry of extracellular calcium into vagal afferent neurons. Increased cytosolic calcium may participate in acute activation of vagal afferent neurons, or it may initiate long-term changes, which modulate future neuronal responses to sensory stimuli.

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Title: Cholecystokinin increases cytosolic calcium in a subpopulation of cultured vagal afferent neurons
Creators: Steven M Simasko - Program in Neuroscience, Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USA. simasko@vetmed.wsu.edu
Jason Wiens
Adrienne Karpiel
Mihai Covasa
Robert C Ritter
Publication Details: American journal of physiology. Regulatory, integrative and comparative physiology, Vol.283(6), pp.R1303-1313
Academic Unit: Integrative Physiology and Neuroscience, Department of
Publisher: United States
Grant note: NS-20561 / NINDS NIH HHS
Identifiers: 99900547045301842
Language: English
Resource Type: Journal article

Cholecystokinin increases cytosolic calcium in a subpopulation of cultured vagal afferent neurons

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