Chromium ( d-Phenylalanine) 3 alleviates high fat-induced insulin resistance and lipid abnormalities

Machender Reddy Kandadi; M.K Unnikrishnan; Ajaya Kumar Sankara Warrier; Min Du; Jun Ren; Nair Sreejayan

doi:10.1016/j.jinorgbio.2010.09.008

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Chromium ( d-Phenylalanine) 3 alleviates high fat-induced insulin resistance and lipid abnormalities

Journal article

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Chromium ( d-Phenylalanine) 3 alleviates high fat-induced insulin resistance and lipid abnormalities

Machender Reddy Kandadi, M.K Unnikrishnan, Ajaya Kumar Sankara Warrier, Min Du, Jun Ren and Nair Sreejayan

Journal of inorganic biochemistry, Vol.105(1), pp.58-62

2011

DOI: https://doi.org/10.1016/j.jinorgbio.2010.09.008

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https://hdl.handle.net/2376/112367

PMCID: PMC3046066

PMID: 21134603

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https://doi.org/10.1016/j.jinorgbio.2010.09.008View

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Abstract

Chromium

Insulin resistance

High-fat diet

High-fat diet has been implicated as a major cause of insulin resistance and dyslipidemia. The objective of this study was to evaluate the impact of dietary-supplementation of chromium ( d-phenylalanine) 3 [Cr( d-Phe) 3] on glucose and insulin tolerance in high-fat diet fed mice. C57BL/6-mice were randomly assigned to orally receive vehicle or Cr( d-Phe) 3 (45 μg of elemental chromium/kg/day) for 8-weeks. High-fat-fed mice exhibited impaired whole-body-glucose and -insulin tolerance and elevated serum triglyceride levels compared to normal chow-fed mice. Insulin-stimulated glucose up-take in the gastrocnemius muscles, assessed as 2-[ 3H-deoxyglucose] incorporation was markedly diminished in high-fat fed mice compared to control mice. Treatment with chromium reconciled the high-fat diet-induced alterations in carbohydrate and lipid metabolism. Treatment of cultured, differentiated myotubes with palmitic acid evoked insulin resistance as evidenced by lower levels of insulin-stimulated Akt-phosphorylation, elevated JNK-phosphorylation, (assessed by Western blotting), attenuation of phosphoinositol-3-kinase activity (determined in the insulin-receptor substrate-1-immunoprecipitates by measuring the extent of phosphorylation of phosphatidylinositol by γ- 32P-ATP), and impairment in cellular glucose up-take, all of which were inhibited by Cr( d-Phe) 3. These results suggest a beneficial effect of chromium-supplementation in insulin resistant conditions. It is likely that these effects of chromium may be mediated by augmenting downstream insulin signaling. Chromium alleviates insulin resistance by inhibiting the deleterious effects of free fatty on insulin signaling pathway. [Display omitted]

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Title: Chromium ( d-Phenylalanine) 3 alleviates high fat-induced insulin resistance and lipid abnormalities
Creators: Machender Reddy Kandadi - School of Pharmacy, Division of Pharmaceutical Sciences, Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, WY 82071, USA
M.K Unnikrishnan - Manipal College of Pharmaceutical Sciences, Manipal University, Karnataka, India
Ajaya Kumar Sankara Warrier - School of Pharmacy, Division of Pharmaceutical Sciences, Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, WY 82071, USA
Min Du - Animal Sciences Department, Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, WY 82071, USA
Jun Ren - School of Pharmacy, Division of Pharmaceutical Sciences, Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, WY 82071, USA
Nair Sreejayan - School of Pharmacy, Division of Pharmaceutical Sciences, Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, WY 82071, USA
Publication Details: Journal of inorganic biochemistry, Vol.105(1), pp.58-62
Academic Unit: Animal Sciences, Department of
Publisher: Elsevier Inc
Identifiers: 99900547312201842
Language: English
Resource Type: Journal article