Chronic alcohol consumption impairs distribution and compromises circulation of B cells in B16BL6 melanoma-bearing mice

Hui Zhang; Zhaohui Zhu; Gary G Meadows

doi:10.4049/jimmunol.1200442

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Chronic alcohol consumption impairs distribution and compromises circulation of B cells in B16BL6 melanoma-bearing mice

Journal article

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Chronic alcohol consumption impairs distribution and compromises circulation of B cells in B16BL6 melanoma-bearing mice

Hui Zhang, Zhaohui Zhu and Gary G Meadows

The Journal of immunology (1950), Vol.189(3), pp.1340-1348

08/01/2012

DOI: https://doi.org/10.4049/jimmunol.1200442

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https://hdl.handle.net/2376/108870

PMCID: PMC3409643

PMID: 22753935

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Abstract

Spleen - immunology

Lymphopenia - pathology

Organ Specificity - immunology

Melanoma, Experimental - immunology

Bone Marrow Cells - immunology

Injections, Subcutaneous

Female

B-Lymphocytes - pathology

T-Lymphocytes - pathology

Disease Models, Animal

Alcohol Drinking - pathology

Bone Marrow Cells - cytology

Lymphopenia - blood

Mice, Inbred C57BL

Melanoma, Experimental - pathology

Spleen - cytology

Lymph Nodes - immunology

Random Allocation

Lymph Nodes - cytology

Alcohol Drinking - adverse effects

Alcohol Drinking - immunology

Animals

B-Lymphocytes - immunology

Cell Line, Tumor

T-Lymphocytes - immunology

Lymphopenia - immunology

Mice

B-Lymphocytes - transplantation

Accumulating research indicates that B cells are involved in anti-tumor immunity. Chronic alcohol consumption is associated with decreased survival of cancer patients. The effect of alcohol consumption on B cells in tumor-bearing hosts is unknown. Results in melanoma-bearing mice showed that chronic alcohol consumption did not alter the percentage and number of B cells in bone marrow, spleen, and lymph nodes but dramatically decreased B cells in the peripheral blood. Alcohol consumption did not alter the development of B cells in the bone marrow and did not affect follicular B cells in the spleen; however, it increased T1 B cells and decreased marginal zone B cells in the spleen. Alcohol consumption also decreased mature B cells in the blood. It did not alter the chemotactic capacity of plasma to facilitate migration of splenocytes or the chemotactic response of splenocytes to CXCL13 and CCL21. However, the response of splenocytes to sphingosine-1-phosphate was impaired in alcohol-consuming, melanoma-bearing mice. The expression of sphingosine-1-phosphate receptor-1 (S1PR1) and sphingosine-1-phosphate lyase-1 (SPL1) in splenocytes was downregulated. Taken together, these results indicate that chronic alcohol consumption decreases peripheral blood B cells by compromising B cell egress from the spleen. The downregulation of S1PR1 and SPL1 expression in alcohol-consuming, melanoma-bearing mice could be associated with compromised egress of B cells from the spleen.

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Title: Chronic alcohol consumption impairs distribution and compromises circulation of B cells in B16BL6 melanoma-bearing mice
Creators: Hui Zhang - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA 99164, USA. hzhang@wsu.edu
Zhaohui Zhu
Gary G Meadows
Publication Details: The Journal of immunology (1950), Vol.189(3), pp.1340-1348
Academic Unit: Pharmaceutical Sciences, Department of
Publisher: United States
Grant note: K05 AA017149 / NIAAA NIH HHS K05-AA017149 / NIAAA NIH HHS R01-AA07293 / NIAAA NIH HHS R01 AA007293 / NIAAA NIH HHS
Identifiers: 99900546893601842
Language: English
Resource Type: Journal article