Journal article
Cloning, Chromosomal Mapping, and Regulatory Properties of the Human Type 9 Adenylyl Cyclase (ADCY9)
Genomics (San Diego, Calif.), Vol.50(1), pp.97-104
05/15/1998
Handle:
https://hdl.handle.net/2376/109139
PMID: 9628827
Abstract
The type 9 adenylyl cyclase (AC9) is a widely distributed adenylyl cyclase that was originally cloned from a mouse cDNA library. Here we report the cloning, chromosomal mapping, and regulatory properties of human AC9 (HGMW-approved symbol ADCY9). Although the human AC9 sequence shows 86% homology with mouse AC9, divergence at the C2a/C2b junction results in an alternative C2b amino acid sequence.In situhybridization localized the human AC9 gene to both human and mouse chromosomes 16. AC9 mRNA is present in all tissues examined, with the highest levels found in skeletal muscle, heart, and brain. To characterize the regulatory properties of human AC9in vivo,the enzyme was expressed in HEK-293 cells. Human AC9 is stimulated by β-adrenergic receptor activation but is insensitive to forskolin, Ca2+and somatostatin. In contrast to mouse AC9, the activity of human AC9 is unaffected by inhibitors of calcineurin. These data emphasize the importance of determining the regulatory properties of human adenylyl cyclases.
Metrics
5 Record Views
Details
- Title
- Cloning, Chromosomal Mapping, and Regulatory Properties of the Human Type 9 Adenylyl Cyclase (ADCY9)
- Creators
- Beth M Hacker - Department of Pharmacology, University of Washington, Seattle, Washington, 98195James E Tomlinson - COR Therapeutics, 256 East Grand Avenue, South San Francisco, California, 94080Gary A Wayman - Department of Pharmacology, University of Washington, Seattle, Washington, 98195Razia Sultana - Department of Pharmacology, University of Washington, Seattle, Washington, 98195Guy Chan - Department of Pharmacology, University of Washington, Seattle, Washington, 98195Enrique Villacres - Department of Pharmacology, University of Washington, Seattle, Washington, 98195Christine Disteche - Department of Pathology, University of Washington, Seattle, Washington, 98195Daniel R Storm - Department of Pharmacology, University of Washington, Seattle, Washington, 98195
- Publication Details
- Genomics (San Diego, Calif.), Vol.50(1), pp.97-104
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547477601842
- Language
- English
- Resource Type
- Journal article